E6 (NC_001587) Virus Tagged ORF Clone

CAT#: VC101919

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041807

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101919 represents NCBI reference of NP_041807 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTTCCCGAACCCTGAGGAACGCCCATACAAACTGCCAGCTTTGTGTGAAGAGGTGAACATTTCAA
  TCCACGAGATCGAGCTTGACTGCGTTTATTGTGAGCGACAACTGTACAGGTGTGAAGTATATGACTTTAT
  TTTTAGAGACCTGTGCGTCGTCTACCGCAAGGGGAAGCCCCTCGGAGTGTGCCAGCCTTGTTTGCTCTTC
  TATTCAAAAGTGCGGCAGTATCGGCGCTACAATCAGAGCGTGTACGGTCGGACACTGGAGAATCTGACCA
  ATAAACAGCTGTGCAATATTTTGATAAGGTGCGGTAAATGCCAGAAACCCCTCTGTCCGTTGGAGAAACA
  AAGACACGTGGATGAAAATAAGCGCTTCCATCAGATAGCAGACCAGTGGACAGGCAGATGCACGCAGTGC
  TGGAGGCCCTCTGCTACCGTCGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101919 representing NP_041807
  Red=Cloning sites Green=Tags
  
  MFFPNPEERPYKLPALCEEVNISIHEIELDCVYCERQLYRCEVYDFIFRDLCVVYRKGKPLGVCQPCLLF
  YSKVRQYRRYNQSVYGRTLENLTNKQLCNILIRCGKCQKPLCPLEKQRHVDENKRFHQIADQWTGRCTQC
  WRPSATVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001587
• ORF Size: 444 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001587.1, NP_041807
• RefSeq ORF: 444
• Locus ID: 1489431
• MW: 17.7 kDa