E1 (NC_001587) Virus Tagged ORF Clone

CAT#: VC101921

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041809

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101921 represents NCBI reference of NP_041809 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACTCTGGAAACTGGGAGGGGCGGTGTAGCGGATGGTTCAATGTTGAGGCAATCGTTGAACGGA
  AAACGGGAGACGCAATTCCAGCGGATGAGAATTATGACGGCGACGACACTGAGGACTCAGAGATGGGTGA
  CTTCATCGACAACGCCCATATCAGCAACATTTACTCTCAGCAGGAAATTGCTCAAGCACTGTACCACAGC
  CAGCAGGTTAACGCTGATAATGAAGCAATTCGAGTGCTGAAAAGAAAGTTTGCAGGCTCTGCTGGGAGCT
  CCCCCGATTCCAAGCGACATGAATTGAAGCACAAGCAAAGATCTCCACATATACTGACCATTAGGGACAC
  AAACACAACCTCTACCCACCTGCTGTGCGAGGAGCAGGATTCCGGATATGGCAACACTGAGGTCGAGACC
  TATGAAAGGCAGGTGCCCGGACCCGGCGGGTGTTTGCAGTCCACATCCTCATCCAACAACGGAAGTCAAA
  TGGCCAGTCCAGGTGAGACGAACTCTGGTAGCAGTAGCATATCCAACATGGATATAGACATGGAGTCCAC
  CCCTATTACTGACATCACCAATATCCTCAAAAGTTCAAACGTGAAAGCTACGCTTCTTGCTAAATTTAAG
  GAAGTCTACGGCCTGAGCTATATGGAGCTGGTGCGCCCCTATAAGTCAGATAAAACACAGTGTCAGGACT
  GGGTGTGCGCTGTCTTCGGTGTGGCGCCGAGCCTCGCCGAGAGTCTGAAAAGCTTGCTGACTCAGTATTG
  CCTGTACATTCACCTCCAGTGTTTGACTTGCAGCTGGGGCATTATCGTGCTGCTGCTGGCCCGGTTCAAG
  TGCAACAAAAATAGACTGACAGTCCAGAAACTCCTGCACGGGCTCCTGAACGTGACACAAGAGTATATGC
  TCATCGAACCTCCAAGATTGAGGTCTACCCCCTGCGCTTTGTACTGGTACCGAACCTCACTGTCAAACAT
  AAGCGAAACAGTAGGGGAGGTACCCGAGTGGATAAAACGGCAGACCGTGGTGCAGCACAGCCTGGAGGAT
  TGTCAGTTTGATCTCTCCCAGATGGTTCAGTGGGCCTTCGATAACGATATTACTAACGATTGCGAAATCG
  CCTACAAGTACGCACTCTTGGCTTCAGAGGACTCTAATGCCGCTGCCTTTCTCAAAAGTAATGCCCAGGC
  CAAGTACGTGAAGGACTGCGGCACAATGTGCCGGCACTACAAGGCCGCTGAACGGAAGCAAATGACCATG
  TCACAATGGATAACACACAGATGCGACCTCATTGATGACGGAGGCAACTGGAAGCATATCGTGCAATTTC
  TCCGCTACCAGCAGGTCGAATTCGTGCCATTTCTGATAGCCTTGAAGCAGTTTCTTAAGGGTATCCCCAA
  GCAGAACTGTATTGTGATCTATGGGCCACCTGACACTGGAAAGTCACACTTCGGCATGAGCCTGATGCAG
  TTCATGCAGGGGGTCGTCATCTCTTACGTAAACTCTAACAGCCACTTTTGGCTGTCTCCTCTGGCCGACG
  CTAAAATGGCTCTGCTTGACGACGCCACTCCGGCCTGTTGGACCTACATCGACAGATATTTGAGGAATGC
  TCTCGACGGAAATCCAATGTGCTTGGATAGGAAACACAAACACCTGTTGCAAATTAAATGCCCACCACTT
  CTGATCACTAGCAATACAAATCCCAAGGCTGATGACACATGGAAATACTTGCACAGTCGAATGAAGGTAT
  TCACCTTTTCTAATCCATTTCCATTCGACTCAAACGGCAACCCACTCTACCAACTGACAAACGAAAACTG
  GAAAGCCTTTTTCACCAAGACTTGGTCAAAACTGGACCTGACAGAGGACGACGATAAAGAGAATGACGGC
  GACACAGTGCAGACCTTTAAGTGCGTTAGTGGCAGGAATCCCCGGACCGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101921 representing NP_041809
  Red=Cloning sites Green=Tags
  
  MADSGNWEGRCSGWFNVEAIVERKTGDAIPADENYDGDDTEDSEMGDFIDNAHISNIYSQQEIAQALYHS
  QQVNADNEAIRVLKRKFAGSAGSSPDSKRHELKHKQRSPHILTIRDTNTTSTHLLCEEQDSGYGNTEVET
  YERQVPGPGGCLQSTSSSNNGSQMASPGETNSGSSSISNMDIDMESTPITDITNILKSSNVKATLLAKFK
  EVYGLSYMELVRPYKSDKTQCQDWVCAVFGVAPSLAESLKSLLTQYCLYIHLQCLTCSWGIIVLLLARFK
  CNKNRLTVQKLLHGLLNVTQEYMLIEPPRLRSTPCALYWYRTSLSNISETVGEVPEWIKRQTVVQHSLED
  CQFDLSQMVQWAFDNDITNDCEIAYKYALLASEDSNAAAFLKSNAQAKYVKDCGTMCRHYKAAERKQMTM
  SQWITHRCDLIDDGGNWKHIVQFLRYQQVEFVPFLIALKQFLKGIPKQNCIVIYGPPDTGKSHFGMSLMQ
  FMQGVVISYVNSNSHFWLSPLADAKMALLDDATPACWTYIDRYLRNALDGNPMCLDRKHKHLLQIKCPPL
  LITSNTNPKADDTWKYLHSRMKVFTFSNPFPFDSNGNPLYQLTNENWKAFFTKTWSKLDLTEDDDKENDG
  DTVQTFKCVSGRNPRTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001587
• ORF Size: 1941 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001587.1, NP_041809
• RefSeq ORF: 1941
• Locus ID: 1489429
• MW: 73.3 kDa