L2 (NC_001587) Virus Tagged ORF Clone

CAT#: VC101923

Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 34], codon optimized for human cell expression, NP_041811

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101923 represents NCBI reference of NP_041811 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGGAAAAGGGATACTCACATCCGACGAAAACGCGCAAGCGCGACTCAGCTGTACAAAACTTGTA
  AGCAAAGCGGTACCTGCCCCCCAGACATCATTCCAAAGGTTGAAGGCAATACCCTGGCAGATCAGATTCT
  TAAGTATGGCAGCATTGGAGTCTTCTTCGGCGGACTGGGTATTGGCTCCGGCTCTGGAACCGGAGGACGC
  ACTGGGTATGTCCCTCTTCCTACGACTACTCCTAGCCGCCCTGTCGAAATCCCTTTGCAGCCAACCCGGC
  CTCCTGTCATTACTTCTGTCGGAGCCAGTGATTCCAGCATCGTGAGTCTGGTAGAGGAATCATCTTTTAT
  TGAGGCTGGCGTCCCCGGCCCCACTAGCATCGTGCCGTCCTCTTCAGGTTTTAACGTCACAACCTCCGTC
  GACTCAACGCCAGCAATCATCGATGTGGCCACCATTTCTGATACCACACAGGTTTCAGTTAGCACCTTTA
  ATAATCCTACGTTTACAGACCCTTCCGTCCTGCAGCCCCCTCCTCCCCTTGAAGCAAGCGGCAGACTGCT
  CTTCTCTAACGACACCGTTACCACCCATTCCTACGAGAACATTCCCCTGGATACCTTCGTGGTCACTACC
  GACAACAACTCTATCGTGAGTAGTACACCCATCCCTGGCAGGCACCCACCTGCCCGCCTGGGGCTTTATG
  GTCGGGCGATTCAGCAAGTAAAAGTGGTCGACCCGGCTTTTGTCACAACGCCTACAAGACTGGTCACTTA
  CGACAACCCTGCTTTCGAAGGCCTGCAAGACACCACACTGGAATTCCAACACAGCGATCTGCACAACGCG
  CCTGATTCCGACTTCCTCGATATAGTGAAGCTGCACCGGCCTGCGCTTACTGCACGCAAGACTGGGATCC
  GCGTTTCACGCCTTGGCCAACGCGCAACTATGTTCACACGGTCTGGAAAAAGGATAGGAGGGAGAGTACA
  CTTCTACCATGACCTGTCTCCCATCCCCACCGAGAATATTGAACTGCAGCCTCTGCTGCCAAGCGCAAGC
  GCAACTGTCACTGATGCCAACGGCATCAACGACGGCCTGTACGACGTGTTGTTGGATAATAATGTGGACA
  TCACGGAGGTGGAAACTCCCACTGGCACCAATACTCAGTCCGTGTTCGCATCAGAGATCAGCACTACCAC
  AGCAAATACGACCATCCCGCTGAATGCAGGGTTGGACACTCACCCCGGCCCAGATATCGCGCTGCCAGTG
  CCAACTGCCGAAACAATCTTCACACCCACCGTGCCGGTGCAACCTAGCGGACCAATCTATATATACGGCA
  GCGATTTCATCCTTCACCCCAGTCTCTATGTGATTCCACGAAAACGCAAGAGACTGTCTTATTTCTTCGC
  CGACGTTGCCACCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101923 representing NP_041811
  Red=Cloning sites Green=Tags
  
  MRRKRDTHIRRKRASATQLYKTCKQSGTCPPDIIPKVEGNTLADQILKYGSIGVFFGGLGIGSGSGTGGR
  TGYVPLPTTTPSRPVEIPLQPTRPPVITSVGASDSSIVSLVEESSFIEAGVPGPTSIVPSSSGFNVTTSV
  DSTPAIIDVATISDTTQVSVSTFNNPTFTDPSVLQPPPPLEASGRLLFSNDTVTTHSYENIPLDTFVVTT
  DNNSIVSSTPIPGRHPPARLGLYGRAIQQVKVVDPAFVTTPTRLVTYDNPAFEGLQDTTLEFQHSDLHNA
  PDSDFLDIVKLHRPALTARKTGIRVSRLGQRATMFTRSGKRIGGRVHFYHDLSPIPTENIELQPLLPSAS
  ATVTDANGINDGLYDVLLDNNVDITEVETPTGTNTQSVFASEISTTTANTTIPLNAGLDTHPGPDIALPV
  PTAETIFTPTVPVQPSGPIYIYGSDFILHPSLYVIPRKRKRLSYFFADVATY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001587
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001587.1, NP_041811
• RefSeq ORF: 1416
• Locus ID: 1489434
• MW: 50.6 kDa