hypothetical protein (NC_001457) Virus Tagged ORF Clone

CAT#: VC101925

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp1 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040889

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101925 represents NCBI reference of NP_040889 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGGACGACCCGCGACTCTGGACGACTTTTGCAGGCGCTTCGACATTAGCTTTTTCGATTTGC
  GCCTTACTTGTATCTTCTGCAGTCACACCGTTGACCTGGCGGATTTGGCTCTGTTCTACTTGAAGAAGCT
  TTCCCTGGTTTTCCGGGGCAACTGCTACTATGCCTGCTGCTCCGAATGCCTCAGACTTTCCGCTCTCTTT
  GAACAAGAAAATTACTTCCAGTGTTCCATTAAGGCCGTCCACCTCGAGGAGATTGCACAGAAAAAAATTA
  AAGAGATTTGCATAAGATGTATCTGTTGCCTGCGGCTGCTTGATATTGTGGAGAAGTTGGATCTCTTGTA
  CTCAGACGAAACCTGCTATCTGATCCGGGGCCTCTGGCGCGGTTATTGCCGAAACTGTATACGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101925 representing NP_040889
  Red=Cloning sites Green=Tags
  
  MADGRPATLDDFCRRFDISFFDLRLTCIFCSHTVDLADLALFYLKKLSLVFRGNCYYACCSECLRLSALF
  EQENYFQCSIKAVHLEEIAQKKIKEICIRCICCLRLLDIVEKLDLLYSDETCYLIRGLWRGYCRNCIRKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001457
• ORF Size: 420 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001457.1, NP_040889
• RefSeq ORF: 420
• Locus ID: 1489450
• MW: 16.5 kDa