hypothetical protein (NC_001457) Virus Tagged ORF Clone

CAT#: VC101929

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp5 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040893

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101929 represents NCBI reference of NP_040893 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCGGGACGAAACTGAAAGTGAAATGGATTATGATGGCCTATACCCTGCAGACCATCCGAGAAA
  AGGAGCTCATCCTGCACTATCTGGCTCTTATGCTGAAAGATCTTGCTGAGCTTGACTGCGGACTGTGCAT
  ACTCAAGCCAAAGCTGTTCCCCCCCCTCCTCCTCGCCCTGCACACGCCACCCCCTTCTACACTGAGGAAC
  AACTCTTACCCAGGGCCACCTCCTGCCACACCTAAACTCCCTTCACGCAGAGCACTCCTGGAGGGCGGGA
  ATCGGGGAAATCCGACACGCCCACCACCACGGCCACTCAAACCGAGAGAATACGACTACGACGAGGACGA
  CGAGAAAGAGAACCAGGGGCCTGGTCAGGAAAAACCCCCAGCCAAAGAAGAAGAAGAGGAGGAGGAAGAG
  GAAGAACGGCCTAACTGGGATCTGCATCATTTGCTGCAGAAGTGGGGTGCGGACATTGACAAACTCAAAG
  ATAAGGTGTGCCGCGATCTCGATAGCTACAAGCAGAAGCTGGGGATTCGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101929 representing NP_040893
  Red=Cloning sites Green=Tags
  
  MNSGTKLKVKWIMMAYTLQTIREKELILHYLALMLKDLAELDCGLCILKPKLFPPLLLALHTPPPSTLRN
  NSYPGPPPATPKLPSRRALLEGGNRGNPTRPPPRPLKPREYDYDEDDEKENQGPGQEKPPAKEEEEEEEE
  EERPNWDLHHLLQKWGADIDKLKDKVCRDLDSYKQKLGIRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001457
• ORF Size: 543 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001457.1, NP_040893
• RefSeq ORF: 543
• Locus ID: 1489454
• MW: 20.8 kDa