E2 (NC_001690) Virus Tagged ORF Clone

CAT#: VC101946

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043419

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101946 represents NCBI reference of NP_043419 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCCGAGACCCAAGAATCACTGTCCACACGCTTCGCTGCTCAGCAGGAGATCCAGCTCACCCTGA
  TAGAGAAGGAAAGCTATGACCTCAAGGATCATCTGGCATACTGGAAAGCCGTGCGGCTCGAAAACGTGAT
  CGCCTACTACGCGCGCAAAGAGCACATTACTAAGCTTGGGCTGCAGCCACTTCCAACGCTGGCTGTGACA
  GAGTATAAAGCTAAGGAGGCGATAAATATCCAGCTGCTTATACAAAGCTTGCTGAAAAGTGAGTTTGCTT
  TGGAGAGATGGACCTTGGCTGAAACCTCCGCTGAAACAATTAATAGCTCACCACGAAACTGTTTTAAAAA
  AGTGCCCTTCATTGTCAATGTTTGGTTTGATAATGACGAAAGAAACAGCTTCCCCTACACGTGCTGGGAC
  TTCATTTACTACCAAGACGACCAGAACAAATGGCATAAGACTGAGGGACTGGTGGACCATAACGGTTGCT
  ACTACGTCGATCTCAATGGAGACTTTGTGTACTTTACATTGTTCCAACCAGACGCGGTGAAGTACGGAAA
  GACCGGTTTGTGGACCGTGCGGTTCAAAAACAAAACTATATCCGCTAGTGTTACCTCATCCAGCAGAAAT
  ACAAATCCCTCTAGCGAGAGCCGCGTCGGCCTGAGCACTTCTAGCAGCTCAGAAAGTCCCCGCAGACGGC
  CAAGTATCTCCGAGAACTCCAACACCGAATCACCAACCTCCTCAACAAGCAGGCTCAGGGAAAGGCGCAG
  AAGAGAGCCCAGAGAATCTGGCACTACTGATACTACTCCCCGGAGGAGAGGTACGAAACGCAAGTTGGGT
  TCCGATAGCGCCCCAACACCCTCTGAGGTGGGCAGTCGAAGTACCACCCTCGCGAGGCACGGCTATTCTC
  GACTTGGACGACTTCAAGAAGAAGCTCGGGATCCTCCTCTGGTGTTGTTCACCGGACAGCAAAATAACCT
  GAAGTGTTGGAGAAATCGGTGCACGACAAAATATGCCTCTCTTTTTTTGTGCTTCAGCAGTGTGTGGAAA
  TGGCTCGGACCAAATTCCGACGGGGGTGCAGCAAAAGTGCTTGTTGCCTTCAAGTCCGATGCTCAACGGC
  AGGTGTTTCTGAATACCGTGCATATCCCTAAGGGCACAACAATCACTCTTGGCCGGCTGGACAGTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101946 representing NP_043419
  Red=Cloning sites Green=Tags
  
  MQPETQESLSTRFAAQQEIQLTLIEKESYDLKDHLAYWKAVRLENVIAYYARKEHITKLGLQPLPTLAVT
  EYKAKEAINIQLLIQSLLKSEFALERWTLAETSAETINSSPRNCFKKVPFIVNVWFDNDERNSFPYTCWD
  FIYYQDDQNKWHKTEGLVDHNGCYYVDLNGDFVYFTLFQPDAVKYGKTGLWTVRFKNKTISASVTSSSRN
  TNPSSESRVGLSTSSSSESPRRRPSISENSNTESPTSSTSRLRERRRREPRESGTTDTTPRRRGTKRKLG
  SDSAPTPSEVGSRSTTLARHGYSRLGRLQEEARDPPLVLFTGQQNNLKCWRNRCTTKYASLFLCFSSVWK
  WLGPNSDGGAAKVLVAFKSDAQRQVFLNTVHIPKGTTITLGRLDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001690
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001690.1, NP_043419
• RefSeq ORF: 1188
• Locus ID: 1403624
• MW: 45.1 kDa