E4 (NC_001690) Virus Tagged ORF Clone

CAT#: VC101947

Myc-DDK-tagged ORF clone of viral ORF for putative, partial [Human papillomavirus type 48], codon optimized for human cell expression, NP_043420

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101947 represents NCBI reference of NP_043420 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  AACATGGAGAAGCTTGACTATGGCCAGCTTGACCTGAAGACCAAGCTGTTCTTGCCCTTGCTCTTGGCCC
  CAAGAGAGATTCAGATTCCATTGTTGAAGGCAGGCTCTGGATCCAGGCCACCCGCCGCCCGACGAGCCTT
  GGAGGGCGACCGAGCATCACAAAAGACTCCCACTCCCTCCAGGCCACCTCCTCGACATCCTGATTATGAA
  AGCGATGACGACGAGAATCGGGAGAACCTCGAACCCCCAACTCCCCACCCCGAGGATGAAGAGCAGCGGG
  GGAATTGGGGGCCTACGCTGCATCAACTCCTGCGGAAATGGGACCAGGACCTGCAGCGCCTTCAGGACAC
  CGTCACACATGACCTGGATGATTACCGCAAGAAATTGGGGATTAGGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101947 representing NP_043420
  Red=Cloning sites Green=Tags
  
  NMEKLDYGQLDLKTKLFLPLLLAPREIQIPLLKAGSGSRPPAARRALEGDRASQKTPTPSRPPPRHPDYE
  SDDDENRENLEPPTPHPEDEEQRGNWGPTLHQLLRKWDQDLQRLQDTVTHDLDDYRKKLGIRH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001690
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001690.1, NP_043420
• RefSeq ORF: 438
• Locus ID: 1403625
• MW: 15.4 kDa