E7 (NC_001593) Virus Tagged ORF Clone

CAT#: VC101972

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 53], codon optimized for human cell expression, NP_041845

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101972 represents NCBI reference of NP_041845 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGCAATGTCCCCACGTTGCCCCAATATATCATCGAGTTGATCCCACAGACTGAGATCGATCTTC
  AGTGTCATGAGCAGTTGAACAGCAGTGAGGACGAAGACGAGGATGAGGTGGACCACCTGCAAGAGCAGCC
  ACAGCAGGCACGACGGGACGAACAGCACCCTTGTTACCTCATTGAGACCCAATGCTGCCGCTGCGAGTCA
  CTTGTTCAACTGGCCGTACAATCTTCCACAAAGGAACTGAGAATTCTTCAACAGATGCTGATGGGGACTG
  TAGAGCTCGTCTGCCCTCTGTGTGCCACCAGGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101972 representing NP_041845
  Red=Cloning sites Green=Tags
  
  MHGNVPTLPQYIIELIPQTEIDLQCHEQLNSSEDEDEDEVDHLQEQPQQARRDEQHPCYLIETQCCRCES
  LVQLAVQSSTKELRILQQMLMGTVELVCPLCATRR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001593
• ORF Size: 315 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001593.1, NP_041845
• RefSeq ORF: 315
• Locus ID: 1489465
• MW: 12.2 kDa