E2 (NC_001593) Virus Tagged ORF Clone

CAT#: VC101973

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 53], codon optimized for human cell expression, NP_041846

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101973 represents NCBI reference of NP_041846 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCTGTGCCAACGCCTCGATGCCTGTCAGGAAAAAATACTCGACTGCTTTGAGCGGGACTCCA
  AAAACATCACCGACCATATCGATTACTGGAAGGCTGTGCGGCAAGAGAATGTCATATATTATAAGGCTAG
  GGAGAACAACATGACCAAACTTGGTCACCAGGTGGTACCTTGCCTGCAGGTGTGTAAGGCCAAGGCATGT
  GTCGCTATCGAGCTTCAGATTGCTCTCGAAAGCCTGTGCAAGACCGAATATAACATGGAAGAATGGACTC
  TCCGGGATGTCTGTGAAAGCATGTGGTACACAGAACCTAAGCAGTGTTTTAAGAAGCAGGGACAACATAT
  CGAGGTTTGGTTCGACGGGTCCAAGGATAACCGGGCCGAATACGTCGTGTGGAAATGGGTGTATTATTGT
  GGAGAGGATGGTTGGTGTAAGGTGTCTTCTGCGGTTAGTTACGAGGGAATCTACTACATTCACGACGGGC
  ACAAGACGTACTACACCAACTTTAAAGACGAGGCCACCAAGTACGGCTGTAAGGGGACTTGGGAAGTGCA
  CATGGGGAAACAGAGTATCTACTGCCCAGACTCCGTGAGTTCTACTTTCAGATCAAACGTGTCATCCGTA
  GAAACCGTGAACGAGTACTATAGTCACAAGACGCCGACAACATCTACACCAGTCGGCACGTACGAAGCTA
  GCTCAAGTCTGAGACCTGGCAAGCGCCCACGAACCACAGAGCCAGATTCAACAGACAGCACGACTCAGAG
  TACTACCACAGCCCGGGAGAGTTATGCAGAGTGCGTAGCTAGAAATACAGATAACACGAACAATAACACA
  CGGAAACACCTCCCCGGGGGCGCATCCTGCAATAATACCGAGATAGATAGCGGCTACAAGACAGCACCAG
  TCGTTCATATTAAGGGCGAGGCTAACAGGCTGAAATGTCTGCGGTACAGGTTCCAGAAGCACAAGCAGCT
  CTTTGTAACTGTGTCCAGTACATATCATTGGACCAACGTTAATTGCGCTGTCAACAACTCCTACATAACA
  GTCGTGTACAAGGACGAAACCCAGCGGCAGAAGTTCCTGGATATTGTGAAAATTCCGCCCAGCGTTTCCC
  TGGTTCTGGGGCACATGACCTGTGTTGACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101973 representing NP_041846
  Red=Cloning sites Green=Tags
  
  METLCQRLDACQEKILDCFERDSKNITDHIDYWKAVRQENVIYYKARENNMTKLGHQVVPCLQVCKAKAC
  VAIELQIALESLCKTEYNMEEWTLRDVCESMWYTEPKQCFKKQGQHIEVWFDGSKDNRAEYVVWKWVYYC
  GEDGWCKVSSAVSYEGIYYIHDGHKTYYTNFKDEATKYGCKGTWEVHMGKQSIYCPDSVSSTFRSNVSSV
  ETVNEYYSHKTPTTSTPVGTYEASSSLRPGKRPRTTEPDSTDSTTQSTTTARESYAECVARNTDNTNNNT
  RKHLPGGASCNNTEIDSGYKTAPVVHIKGEANRLKCLRYRFQKHKQLFVTVSSTYHWTNVNCAVNNSYIT
  VVYKDETQRQKFLDIVKIPPSVSLVLGHMTCVDM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001593
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001593.1, NP_041846
• RefSeq ORF: 1152
• Locus ID: 1489466
• MW: 44.2 kDa