L1 (NC_001593) Virus Tagged ORF Clone

CAT#: VC101975

Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 53], codon optimized for human cell expression, NP_041848

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101975 represents NCBI reference of NP_041848 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGTGGCGGCCCAGCGATTCAAAGGTGTACCTCCCACCTACCCCTGTCTCCAAGGTCATAACCA
  CTGATGCATACGTGAAGCGGACCACCATTTTCTATCACGCTGGATCATCACGACTGCTGACCGTGGGACA
  TCCATACTATCCTATCAGCAAGTCCGGAAAAGCAGACATCCCCAAGGTGTCCGCCTTTCAATACAGGGTT
  TTTCGGGTGCGGCTTCCCGACCCGAATAAATTTGGACTGCCTGATACAAATATTTTCAATCCTGATCAGG
  AGCGGCTCGTCTGGGCATGCGTTGGCCTTGAGATTGGGAGGGGGCAGCCCCTCGGCGTCGGTGTTAGCGG
  CCACCCTCTCTTTAACCGACTGGACGACACCGAGTCCAGCAGTATCGCCATACAGGACACAGCCCCAGAT
  TCAAGAGATAATGTCAGTGTGGATCCCAAGCAAACCCAACTGTGCATTATTGGTTGCGCCCCAGCCATCG
  GCGAGCATTGGACTAAAGGCACTGCCTGTAGGTCAACTCCAACCACCGCAGGTGACTGCCCACCCCTTGA
  ACTTATCAACTCCCCTATTGAGGATGGGGATATGGTCGACACAGGATTTGGCGCCCTCAACTTTAAGGCC
  CTGCAGGAGTCCAAGAGCGATGTCCCCCTCGACATCGTTCAGTCTACTTGCAAGTATCCAGACTATCTGA
  AAATGAGCGCTGACGCCTATGGGGACAGCATGTGGTTTTACCTGAGACGGGAGCAGCTTTTCACTAGGCA
  CTTCTTCAATCGGGCCGGCGTCATTGGCGAGGAGATTCCTAACGACCTGTACATAAAGGGGAGTAATGGT
  AGGGACCCCCCTCCCTCCTCCGTGTATGTCGCCACTCCTAGCGGCTCCATGATTACTAGCGAAGCACAGC
  TGTTCAACAAGCCGTACTGGCTGCAGAGGGCACAGGGCCACAACAATGGCATCTGCTGGAACAATCAACT
  CTTTGTTACTGTCGTCGATACTACCAGAAACACAAACATGACCTTGTCTGCAACCACCCAATCAATGTCC
  ACATACAACTCCAAGCAGATTAAACAGTATGTGAGGCACGCTGAGGAATACGAGCTGCAGTTCGTATTTC
  AACTCTGTAAGATATCCCTGTCCGCTGAAGTGATGGCCTATCTCCATACTATGAACTCAACTCTCCTTGA
  AGATTGGAACATCGGACTCTCCCCTCCCGTAGCCACAAGCCTCGAAGATAAATACAGATATGTGAAATCC
  GCCGCCATTACTTGCCAGAAGGACCAGCCCCCTCCTGAGAAGCAGGATCCACTTAGCAAGTACAAATTCT
  GGGAAGTGAATCTTCAGAACAGCTTCTCTGCCGACTTGGACCAATTCCCTCTCGGAAGAAAGTTTCTCAT
  GCAGGTGGGAGTCAGAACCAAACCCCCAGTTTCCTCCAAAAAACGCAGTGCTTCAACAACCAGCACATCA
  GCTCCAAGCTCTAAAAGGAAAAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101975 representing NP_041848
  Red=Cloning sites Green=Tags
  
  MAVWRPSDSKVYLPPTPVSKVITTDAYVKRTTIFYHAGSSRLLTVGHPYYPISKSGKADIPKVSAFQYRV
  FRVRLPDPNKFGLPDTNIFNPDQERLVWACVGLEIGRGQPLGVGVSGHPLFNRLDDTESSSIAIQDTAPD
  SRDNVSVDPKQTQLCIIGCAPAIGEHWTKGTACRSTPTTAGDCPPLELINSPIEDGDMVDTGFGALNFKA
  LQESKSDVPLDIVQSTCKYPDYLKMSADAYGDSMWFYLRREQLFTRHFFNRAGVIGEEIPNDLYIKGSNG
  RDPPPSSVYVATPSGSMITSEAQLFNKPYWLQRAQGHNNGICWNNQLFVTVVDTTRNTNMTLSATTQSMS
  TYNSKQIKQYVRHAEEYELQFVFQLCKISLSAEVMAYLHTMNSTLLEDWNIGLSPPVATSLEDKYRYVKS
  AAITCQKDQPPPEKQDPLSKYKFWEVNLQNSFSADLDQFPLGRKFLMQVGVRTKPPVSSKKRSASTTSTS
  APSSKRKRK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001593
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001593.1, NP_041848
• RefSeq ORF: 1497
• Locus ID: 1489468
• MW: 55.7 kDa