E2 (NC_001355) Virus Tagged ORF Clone

CAT#: VC101995

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040299

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101995 represents NCBI reference of NP_040299 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCATCGCTAAAAGACTTGACGCTTGCCAGGAACAACTCCTGGAGCTGTACGAAGAGAACTCCA
  CTGATCTGCACAAGCACGTCCTGCATTGGAAATGTATGCGCCATGAGAGCGTGCTGCTCTATAAAGCAAA
  ACAGATGGGCCTTTCACACATCGGCATGCAAGTGGTTCCCCCCCTCAAAGTTTCTGAGGCTAAGGGTCAC
  AATGCCATCGAAATGCAGATGCACCTCGAGAGCCTGCTGCGGACCGAATATTCCATGGAACCATGGACCC
  TGCAAGAAACTAGCTATGAGATGTGGCAAACCCCGCCAAAACGGTGCTTTAAGAAGAGAGGCAAAACAGT
  GGAGGTGAAATTTGACGGGTGCGCCAACAATACAATGGATTACGTAGTCTGGACTGATGTCTATGTGCAG
  GATAACGACACATGGGTGAAAGTGCATTCTATGGTAGACGCTAAAGGCATATACTACACTTGTGGGCAGT
  TCAAGACCTACTATGTCAATTTTGTTAAGGAAGCAGAAAAATATGGGAGTACAAAGCACTGGGAAGTCTG
  CTATGGTTCTACCGTGATCTGCAGCCCCGCTTCCGTATCTTCTACAACCCAAGAGGTCTCTATTCCTGAG
  TCAACAACCTATACCCCCGCCCAGACTAGCACTCTTGTGAGCTCAAGTACAAAAGAGGATGCGGTGCAGA
  CCCCACCAAGAAAGAGAGCCAGGGGTGTGCAGCAGTCTCCGTGTAACGCTCTCTGCGTGGCCCATATCGG
  CCCTGTGGATTCTGGCAACCACAACCTCATCACGAATAATCATGACCAGCACCAGAGACGGAATAACTCT
  AACTCCTCCGCCACTCCAATCGTGCAGTTTCAAGGGGAATCTAATTGCCTGAAGTGTTTCAGATACAGGC
  TGAATGACCGGCATCGCCACCTGTTCGACCTCATTAGCAGCACCTGGCACTGGGCGTCTAGCAAAGCACC
  TCACAAACATGCGATCGTGACAGTCACCTATGATTCCGAAGAACAGCGCCAGCAGTTCTTGGATGTGGTG
  AAGATTCCCCCCACCATCAGCCATAAGCTGGGGTTTATGTCACTCCACCTTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101995 representing NP_040299
  Red=Cloning sites Green=Tags
  
  MEAIAKRLDACQEQLLELYEENSTDLHKHVLHWKCMRHESVLLYKAKQMGLSHIGMQVVPPLKVSEAKGH
  NAIEMQMHLESLLRTEYSMEPWTLQETSYEMWQTPPKRCFKKRGKTVEVKFDGCANNTMDYVVWTDVYVQ
  DNDTWVKVHSMVDAKGIYYTCGQFKTYYVNFVKEAEKYGSTKHWEVCYGSTVICSPASVSSTTQEVSIPE
  STTYTPAQTSTLVSSSTKEDAVQTPPRKRARGVQQSPCNALCVAHIGPVDSGNHNLITNNHDQHQRRNNS
  NSSATPIVQFQGESNCLKCFRYRLNDRHRHLFDLISSTWHWASSKAPHKHAIVTVTYDSEEQRQQFLDVV
  KIPPTISHKLGFMSLHLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001355
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001355.1, NP_040299
• RefSeq ORF: 1104
• Locus ID: 1489364
• MW: 42.2 kDa