E5b (NC_001355) Virus Tagged ORF Clone

CAT#: VC101998

Myc-DDK-tagged ORF clone of viral ORF for E5b protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040302

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E5b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101998 represents NCBI reference of NP_040302 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTCACCTGCCAGTTTAACGACGGCGACACTTGGCTGGGTCTGTGGCTTCTGTGCGCTTTTATTG
  TAGGCATGCTGGGATTGCTGCTGATGCATTATAGAGCCGTGCAGGGGGACAAGCACACGAAGTGTAAAAA
  GTGCAACAAACACAACTGTAACGACGATTATGTGACAATGCATTACACTACAGATGGGGACTATATCTAT
  ATGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101998 representing NP_040302
  Red=Cloning sites Green=Tags
  
  MMLTCQFNDGDTWLGLWLLCAFIVGMLGLLLMHYRAVQGDKHTKCKKCNKHNCNDDYVTMHYTTDGDYIY
  MN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001355
• ORF Size: 216 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001355.1, NP_040302
• RefSeq ORF: 216
• Locus ID: 1489367
• MW: 8.4 kDa