E4 (NC_001355) Virus Tagged ORF Clone

CAT#: VC101996

Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040300

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101996 represents NCBI reference of NP_040300 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCCCTAATATCGGCAAGTATGTCATGGCAGCGCAGCTCTATGTGCTGCTTCACCTGTATCTGG
  CCCTCCACAAGAAGTATCCTTTTCTCAACCTCCTGCATACCCCTCCCCACCGCCCCCCTCCGCTCTGTCC
  ACAGGCACCACGCAAAACTCAATGTAAGCGGAGACTGGGAAATGAGCACGAAGAAAGCAACTCTCCTCTC
  GCTACCCCTTGTGTCTGGCCCACCCTTGACCCATGGACAGTGGAAACGACTACTTCTAGTCTTACGATAA
  CTACCAGCACTAAAGATGGCACCACCGTGACAGTACAGCTGCGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101996 representing NP_040300
  Red=Cloning sites Green=Tags
  
  MGAPNIGKYVMAAQLYVLLHLYLALHKKYPFLNLLHTPPHRPPPLCPQAPRKTQCKRRLGNEHEESNSPL
  ATPCVWPTLDPWTVETTTSSLTITTSTKDGTTVTVQLRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001355
• ORF Size: 327 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001355.1, NP_040300
• RefSeq ORF: 327
• Locus ID: 1489365
• MW: 12.2 kDa