E1 (NC_010329) Virus Tagged ORF Clone

CAT#: VC102009

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672010

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102009 represents NCBI reference of YP_001672010 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCCAAATAAAGGAACAGACACCACCGATAACTTCGATTGCAACTCAGAGTGGCTCCTGGTCG
  ATGAGGCGGAATGCGTCGATGACCTGGATGTGTTGGATGAGCTGCTGGAAGAAAGTACTCAGTGTAGCAC
  TGTCAGCAATCTCATAGACGATGACCCCGTAGACATCGAAGACCAGGGTAACTCCCTCGCTCTTTTCAAT
  TCACAGGTGACCGAGGACTGTAATACTGCAATCTCCGCACTCAAGAGAAAGCTGATCAAGAGCCCTCAGC
  TCCAGACCGTGGCTGAGCTTAGTCCGAGATTGCAGGCTGTGAGCATTAGTCCCAAAAAACAGTCTAAACG
  CCGCCTGTTCGATGACTCCGGCATAGGGGAAGACGAAGCCCAGAACACATCACAGGTGGCCCTGGACAGC
  CTGGAATCCTCATTGGAAACAAGCGGGGCCGGCCTTACGATTAACCTGCTTCACTGTAGTAATAGACAGG
  CCACTGTGCATTTGAAATTCAAAGAGCTCTATGGAGTGTCATTCAAGGAACTGACGAGAAACTTCAAGTC
  CGATAAGTCCTGCAGTAACTCATGGGTAATCGCTGTGTACAATGCAGTTGAAGAAGTCCTGGAAGCATCT
  AAAGTTCAGCTGCAGAAGCATGTCGAGTTCTTGCAGCTTATCGTGTACGGATTCCATGGACTTTATCTGA
  TTGTGTTTAAGAGCACCAAGAACCGCGACACAGTGTCAAAACTTTTCACCGAAATGCTTAACGTCAATGC
  TCTTCAGCTGATGTGTGACCCACCCAGGACACGCTCAGTGCCTGTCGCATTGTTCTTTTACCGCAAGTCC
  CTGGGCAACGCGAGTTTTATGTACGGTGAGGTCCCAGATTGGATCAAGAAGCAGACCCTGGTCGAACACC
  AGTCAGCCTCCGCCGCCGAGACCTTCGATTTCTCTTCAATGGTGCAGTGGGCTTACGATAATGAATTCAC
  TGAAGAGGCCGAGATCGCTTATCAATATGCCCTCGCCGCAGACGAAGACCCTAACGCAGAAGCTTTCTTG
  AAGCATAATAACCAGTACAAATTCGTCAAGGATTGCGCTGCCATGGTCAAAATGTATAGACGGTACGAAC
  TCAGACAGATGTCTATGGCAGAGTGGATTGATAAATGCTGTTCAAAATGCACCGAAAATGGAAATTGGAA
  AATCATTGCGACATTCCTCAAACACCAGAACGTACAGTTTCTCTCCTTTCTTATTGCCCTGAAACCCTTC
  CTTCAGGGGGTGCCTAAGAAAAATTGCCTGGTGTTCGTCGGCCCACCTGACACTGGCAAGAGCTATTTCT
  GTTTTAGCCTTATCCGCTTTTTCGGGGGCAAAGTGGTGTCCTTCATGAACAGAAACAGTCATTTTTGGCT
  GCAGCCTCTGCAGGATACCAAATTGGGATTCCTGGACGACGCTACCCATCCCTGTTGGGTGTACATGGAC
  ATCAACCTGCGGAACGGCCTGGATGGCAACCTTGTCTCCCTTGATGCGAAACACCGCGCCCCGATGCAGA
  TGAAACTGCCACCATTGTTGGTGACAACTAATGTGGACGTCATGGAAGATCAATCACTGAGATATCTGCA
  TTCCCGCCTGGTATGCTTCAAATTTCCAAATAAACTCCCACTGAATGATGACGGCAGCCTGGTCTACGAG
  ATCACAAACGACACATGGGCTTGCTTCTTTCGCAAATTCGCTACCCAGCTGAACTTGGTGCTCGAGGATG
  GCGAGAATGGCAATACAGGTAACTCAGACCGCACATTTCGCTGCACTGCCAGATGCCATATCGAGTCAAA
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102009 representing YP_001672010
  Red=Cloning sites Green=Tags
  
  MADPNKGTDTTDNFDCNSEWLLVDEAECVDDLDVLDELLEESTQCSTVSNLIDDDPVDIEDQGNSLALFN
  SQVTEDCNTAISALKRKLIKSPQLQTVAELSPRLQAVSISPKKQSKRRLFDDSGIGEDEAQNTSQVALDS
  LESSLETSGAGLTINLLHCSNRQATVHLKFKELYGVSFKELTRNFKSDKSCSNSWVIAVYNAVEEVLEAS
  KVQLQKHVEFLQLIVYGFHGLYLIVFKSTKNRDTVSKLFTEMLNVNALQLMCDPPRTRSVPVALFFYRKS
  LGNASFMYGEVPDWIKKQTLVEHQSASAAETFDFSSMVQWAYDNEFTEEAEIAYQYALAADEDPNAEAFL
  KHNNQYKFVKDCAAMVKMYRRYELRQMSMAEWIDKCCSKCTENGNWKIIATFLKHQNVQFLSFLIALKPF
  LQGVPKKNCLVFVGPPDTGKSYFCFSLIRFFGGKVVSFMNRNSHFWLQPLQDTKLGFLDDATHPCWVYMD
  INLRNGLDGNLVSLDAKHRAPMQMKLPPLLVTTNVDVMEDQSLRYLHSRLVCFKFPNKLPLNDDGSLVYE
  ITNDTWACFFRKFATQLNLVLEDGENGNTGNSDRTFRCTARCHIESN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010329
• ORF Size: 1821 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010329.1, YP_001672010
• RefSeq ORF: 1821
• Locus ID: 5896661
• MW: 68.9 kDa