E2 protein (NC_010329) Virus Tagged ORF Clone

CAT#: VC102010

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672011

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102010 represents NCBI reference of YP_001672011 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCATGGAGACCCAGGAGACCCTGACTGAGAGATTCGTCGCCCTGCAAGATGCTATACTTAATC
  TGATCGAAAGAGGAGAAACGGATCTGCGGAGTCAGATACAGTACTGGGAACTGGTTCGAAAAGAACAGGT
  GATCCTCTATTATGCTCGGAAATCCGGCTACAACCGCCTCGGGCTGCAGCCTACTCCTGCCCCTGCTGTG
  TCAGAATATAACGCCAAGCAGGCCATCCACCTCCAGCTGATGCTGAAATCTTTGGAGAAATCAAAATTCG
  CTAAGGAGCCTTGGTCACTGACCGACGCCAGCGCCGAGTTGGTGAATACACCCCCTAGAGACTGTTTCAA
  AAAGGGAGGGTTCACAGTCACTGTGTATTTCGATAACGACAGGGAGAACAGCTTCCCTTATACTCAATGG
  GAGCATATCTACTACCAGGATCAAAATGAGCAGTGGCACAAAGTCCCCGGAGGAGTTGACCATAATGGCC
  TGTATTACGATGAGGAGAACACGAATGAACGGGTGTATTTCCTGCTGTTTGAGCCTGAGAGCCAGAAATA
  CGGCTCAAGCGGACAGTGGACTGTACACTACAAGAATACGACTGTGAGCGCTAGTGCTACTTCCAGCTCC
  AGACGGAGTAGTCCTATTAGTACAAAGACTGACTTTGACGCCACCACAGCTGGGAACACGACAACCTCCG
  CACCCCAAAGATCTCCGAGAAAGAGGCTGCAGGAAGCCGTATCATCCACAACATCCCCCCCAGCCCATAA
  TCTTCGGTCTCCCGGACGGGGTCGCGGGGAGGGCGAGCGGACAAGTGGAGCTAAGCGAAGAAGAACCGCC
  ACCGATGGAAACACCCTGGGAGAATCAGTCCCCTCCCCATCCCAGGTGGGATCCAGGCATAGGGCACCCG
  AGCGCTCCGGCCTGTCTCGACTCGGCCGCCTTCAGGCAGACGCTTGGGATCCACCACTTATAATCATTAA
  GGGGCCTGCGAATACTCTTAAATGTTGGAGAAATCGGATGAAGAAGAATAGCTCCTCTAACCTGGTCTGC
  AGTTCCGTGTGGCGGTGGATAGACTCAACGACACACGAAAACTCCAGAATGTTGGTGGCGTTTCAAAATA
  CCGCGGAAAGGACACGCTTCCTTAACTCTGTCACACTTCCCAAGGGGACCACATATGCCTTCGGCTACCT
  GGACTCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102010 representing YP_001672011
  Red=Cloning sites Green=Tags
  
  MARMETQETLTERFVALQDAILNLIERGETDLRSQIQYWELVRKEQVILYYARKSGYNRLGLQPTPAPAV
  SEYNAKQAIHLQLMLKSLEKSKFAKEPWSLTDASAELVNTPPRDCFKKGGFTVTVYFDNDRENSFPYTQW
  EHIYYQDQNEQWHKVPGGVDHNGLYYDEENTNERVYFLLFEPESQKYGSSGQWTVHYKNTTVSASATSSS
  RRSSPISTKTDFDATTAGNTTTSAPQRSPRKRLQEAVSSTTSPPAHNLRSPGRGRGEGERTSGAKRRRTA
  TDGNTLGESVPSPSQVGSRHRAPERSGLSRLGRLQADAWDPPLIIIKGPANTLKCWRNRMKKNSSSNLVC
  SSVWRWIDSTTHENSRMLVAFQNTAERTRFLNSVTLPKGTTYAFGYLDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010329
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010329.1, YP_001672011
• RefSeq ORF: 1200
• Locus ID: 5896663
• MW: 45.3 kDa