L2 protein (NC_010329) Virus Tagged ORF Clone

CAT#: VC102012

Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Human papillomavirus type 88], codon optimized for human cell expression, YP_001672013

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102012 represents NCBI reference of YP_001672013 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAAGACCCGGTCCAAACGGGATACCGCCGAGAACTTGTATCGGCATTGTAAAGCTACCGGAAACT
  GTCCTCCTGATGTAGAGAATAAAATTGAAGGCAACACCCTCGCCGATCGGCTTCTGCGCATCTTTGGATC
  AGTCATCTATCTTGGCGGCCTTGGCCTGGGTACAGGGGAAGGAACCACAGGTATCCGACCCATTGAGGCC
  CCTGTTGAGACCGTAAGGCCCGACATTACTGTGGAACGGCCCACCGTACGACCCCGGCCGCAGAGACCCA
  CGACATTTGGCACCCCTATAGATCGGATCGGCTCTGCTGACATTACTCCTAATGTCGTGAAACCCACTGA
  GAGTTCCATCGTGCCATTGAATGAGAGTGGCATTCCTGATCCCACTATTATCGATTCCGCTACGGGGGGT
  GGAGAAGGGTTGGGGGAATACGATATTCTTACAACTGTGGACCCCAATGAAACACTGGGCGCTACCGGCG
  GTCACCCAACAACTAGCGGGACTCTGAATAATGAGACGGCGATCCTGGACATTTCACCATATGAGCCACC
  CCCAAAAAGATTTGCTCTGGCACCTAGCGTTCACGCTGAAGCAGACATTACCATCATTGAGTCATCACTT
  CCCACAGAAAGCAACATTAATGTGTTTGTGGACGCCAACATTACTGGGGAGATCGTGGGGGAGGAAATTC
  CCCTCGAGCCAATCAACAGTATCGAAGAATTCGAAATTGAGGCCGGCAGACAGACCTCCACACCTCGAGA
  AGCTGTCGAAAGGTTCCTCGGCAGAGCCAGAAGTCTCTACAACCGCTACATCCAACAGATCCGCACCGAT
  AATGTGGACTTCCTCACGCGGCCTTCACGCGCTGTGCAGTTCGAATTTGAAAATCCAGCTTTCACCGGAG
  ACGTCAGCCTGGAATTCGCAAGGGACGTGGCAGAAATCACCGCAGCACCCGATCCCGATTTTGCCGACAT
  AATCCGACTTGGCCGGCCAATATTCTCCGAAACTCCCGGAGGTACAGTGCGAGTGAGCCGGCTTGGCACA
  AAAGGCGCCATCAGCACAAGATCCGGAACAATCATCGGACCACGGGTGCACTACTATTTTGATCTCTCCG
  CCATTGAGCCCATCGAACCGGATGTAATCGAGCTCTCAAATTTGGGGGAGTTTAGTGGTGAATCCACTAT
  AGTGGATTCTATTCTCAGCGGCCATACAGTGGATCCCATAGCACCCTTCGAATCAACCTTTTCAATTGCA
  GACCTTGAGGACCCCCTTCTCGAGGATTTTAGCAACTCCCATTTGTTCGTTCATTTCGAGGAAGAAGATG
  AATTGATTTCTGTGCCTACACTGCCTCCCGGAGCCGCAATCAAAGCTTTTGTAGATGATTATGCAGACAT
  TATCGTGAGCTACCCCGAGATGGTGAACGTGAATAAAATTGAGATACCTGCTACTACTTTGGTTCCCTCC
  GAGCCAGATATTCGGCTGGACTGGTTCAGCCCAGACTACGATCTGCACCCCAGCCTTCTGCGGCGGAGAA
  GGAAGCGCAAGAGGAATATGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102012 representing YP_001672013
  Red=Cloning sites Green=Tags
  
  MYKTRSKRDTAENLYRHCKATGNCPPDVENKIEGNTLADRLLRIFGSVIYLGGLGLGTGEGTTGIRPIEA
  PVETVRPDITVERPTVRPRPQRPTTFGTPIDRIGSADITPNVVKPTESSIVPLNESGIPDPTIIDSATGG
  GEGLGEYDILTTVDPNETLGATGGHPTTSGTLNNETAILDISPYEPPPKRFALAPSVHAEADITIIESSL
  PTESNINVFVDANITGEIVGEEIPLEPINSIEEFEIEAGRQTSTPREAVERFLGRARSLYNRYIQQIRTD
  NVDFLTRPSRAVQFEFENPAFTGDVSLEFARDVAEITAAPDPDFADIIRLGRPIFSETPGGTVRVSRLGT
  KGAISTRSGTIIGPRVHYYFDLSAIEPIEPDVIELSNLGEFSGESTIVDSILSGHTVDPIAPFESTFSIA
  DLEDPLLEDFSNSHLFVHFEEEDELISVPTLPPGAAIKAFVDDYADIIVSYPEMVNVNKIEIPATTLVPS
  EPDIRLDWFSPDYDLHPSLLRRRRKRKRNMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010329
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010329.1, YP_001672013
• RefSeq ORF: 1563
• Locus ID: 5896665
• MW: 57.1 kDa