VP2 (NC_009996) Virus Tagged ORF Clone

CAT#: VC102131

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human rhinovirus C], codon optimized for human cell expression, YP_001552433

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102131 represents NCBI reference of YP_001552433 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCAACTGTTGAGGCCTGTGGCATGTCCGATAGGCTCAAACAGATCACTATCGGAAATTCAACAA
  TCACCACCCAGGATACTCTCAACTCCATTCTCGCATACGGGGAATGGCCAAAGTACCTGTCTGATCTTGA
  TGCCTCCTCCGTGGACAAGCCAACCCACCCGGAAACTTCCTCTGATCGCTTCTACACACTCACATCAGTA
  GATTGGACTACTACCTCAAAGGGTTGGTGGTGGAAGCTCCCCGATTGTCTGAAAGACATGGGAATCTTTG
  GCCAAAATCTGTATCATCACGCACTGGGTAGGTCAGGCTATATTATTCACACACAGTGTAACGCTACAAA
  ATTCAATTCCGGCTGCCTGATCGTGGCAGTGGTCCCCGAGCACCAGCTCGCATATATCGGTGAAGCTAAC
  GTAAACGTGGGATACGACCACACACACCCCGGAGAAGGGGGACATGTAATCGGGAGCAACGTTCGGAGGG
  ACAATAAACAGCCCGATGAAGACCCTTTCTTCAATTGCAATGGCACCCTCCTGGGTAATATAACCATCTT
  TCCACACCAGCTTATAAATCTGCGGACGAACAACTCTTCCACCATAGTCGTCCCCTACATAAACTGCGTC
  CCTATGGATAATATGCTGAGGCATAACAACCTTAGCTTGGTGATTATCCCAATCGTCCCACTGAGAGCCG
  CCAACGGAGTGACAAAAGTGCCGATCACGATCAGCATCGCCCCAGACAAATCTGAATTCTCCGGCGCGAG
  ACAGAGCGTCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102131 representing YP_001552433
  Red=Cloning sites Green=Tags
  
  MSPTVEACGMSDRLKQITIGNSTITTQDTLNSILAYGEWPKYLSDLDASSVDKPTHPETSSDRFYTLTSV
  DWTTTSKGWWWKLPDCLKDMGIFGQNLYHHALGRSGYIIHTQCNATKFNSGCLIVAVVPEHQLAYIGEAN
  VNVGYDHTHPGEGGHVIGSNVRRDNKQPDEDPFFNCNGTLLGNITIFPHQLINLRTNNSSTIVVPYINCV
  PMDNMLRHNNLSLVIIPIVPLRAANGVTKVPITISIAPDKSEFSGARQSVKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009996
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009996.1, YP_001552433
• RefSeq ORF: 786
• MW: 28.9 kDa