3A (NC_009996) Virus Tagged ORF Clone

CAT#: VC102137

Myc-DDK-tagged ORF clone of viral ORF for 3A [Human rhinovirus C], codon optimized for human cell expression, YP_001552439

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102137 represents NCBI reference of YP_001552439 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCGGGGATAAGGAGACACCTGGGTTCATCATAGATCTGCTGTCCTCTAGTAAAGATCCCAAAG
  TCATTCAATACTGCCAGGACCAAGGCTACATCTCTCCCGTGCATGGTTCCATAGAACGAGACTTTGCGTA
  CGTGACTTACGCTTTGAATTGCCTGGGGTCTCTGGTGATCATCCTCGGAACGATTTATGCCCTGTACAGG
  CTGATGTGCATAGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102137 representing YP_001552439
  Red=Cloning sites Green=Tags
  
  MGLGDKETPGFIIDLLSSSKDPKVIQYCQDQGYISPVHGSIERDFAYVTYALNCLGSLVIILGTIYALYR
  LMCIAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009996
• ORF Size: 228 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009996.1, YP_001552439
• RefSeq ORF: 228
• MW: 8.4 kDa