E1 protein (NC_004102) Virus Tagged ORF Clone

CAT#: VC102216

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751920

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102216 represents NCBI reference of NP_751920 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAGGTCAGAAATAGCTCTGGGCTGTATCACGTGACAAATGATTGCCCGAACAGCTCTATTGTCT
  ATGAGGCCGCTGACGCAATTCTCCACACCCCTGGCTGTGTGCCTTGTGTTCGGGAGGGCAATGCCTCTCG
  CTGTTGGGTTGCCGTCACACCCACCGTCGCCACACGCGATGGAAAGCTGCCCACAACGCAGCTGAGGCGG
  CACATTGACCTTCTGGTCGGTTCAGCTACGCTGTGCTCAGCCCTGTACGTCGGAGACCTGTGTGGCAGCG
  TGTTTCTGGTCGGACAGCTCTTTACCTTCTCTCCAAGACGGCATTGGACCACCCAGGATTGTAACTGCAG
  CATTTACCCGGGTCATATAACCGGCCATCGCATGGCTTGGGACATGATGATGAATTGGTCTCCTACGGCC
  GCCCTGGTGGTTGCCCAATTGCTTAGGATCCCCCAGGCGATCATGGACATGATTGCTGGAGCCCATTGGG
  GCGTTCTCGCGGGAATCGCCTACTTCAGTATGGTAGGGAACTGGGCAAAGGTGCTTGTGGTGTTGTTGTT
  GTTTGCCGGCGTTGACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102216 representing NP_751920
  Red=Cloning sites Green=Tags
  
  MYQVRNSSGLYHVTNDCPNSSIVYEAADAILHTPGCVPCVREGNASRCWVAVTPTVATRDGKLPTTQLRR
  HIDLLVGSATLCSALYVGDLCGSVFLVGQLFTFSPRRHWTTQDCNCSIYPGHITGHRMAWDMMMNWSPTA
  ALVVAQLLRIPQAIMDMIAGAHWGVLAGIAYFSMVGNWAKVLVVLLLFAGVDA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004102
• ORF Size: 579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004102.1, NP_751920
• RefSeq ORF: 579
• MW: 21.0 kDa