E2 protein (NC_004102) Virus Tagged ORF Clone

CAT#: VC102217

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751921

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102217 represents NCBI reference of NP_751921 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCATGTTACAGGGGGCAGCGCAGGCAGGACCACAGCTGGCCTCGTAGGGCTCCTGACCCCTG
  GTGCCAAGCAGAACATTCAGCTTATTAACACAAATGGCTCCTGGCATATAAACTCTACAGCACTGAATTG
  CAATGAGAGTCTTAATACGGGCTGGCTGGCAGGCCTCTTTTACCAACACAAGTTTAACTCTTCCGGATGT
  CCTGAAAGGTTGGCCAGCTGTCGCCGACTCACCGACTTTGCCCAGGGTTGGGGTCCTATCTCTTACGCCA
  ACGGGTCTGGACTCGATGAGCGCCCCTACTGCTGGCATTACCCGCCACGACCCTGCGGCATTGTGCCGGC
  AAAAAGTGTTTGCGGTCCCGTGTATTGCTTTACTCCATCCCCCGTGGTCGTCGGCACTACCGATCGGAGT
  GGCGCCCCTACATACTCATGGGGGGCTAACGACACCGACGTTTTTGTATTGAACAATACCAGGCCCCCTT
  TGGGCAACTGGTTTGGGTGTACTTGGATGAATTCTACAGGCTTCACGAAAGTGTGCGGAGCACCACCTTG
  CGTCATCGGGGGTGTGGGTAATAATACTCTGCTGTGCCCAACAGACTGTTTCCGGAAACACCCTGAGGCT
  ACGTATAGTAGGTGCGGCTCCGGCCCTTGGATAACACCTCGCTGCATGGTTGACTACCCCTATCGCCTTT
  GGCACTACCCATGTACAATCAACTATACCATTTTTAAGGTACGAATGTACGTCGGCGGCGTTGAACACCG
  GCTGGAGGCGGCATGTAACTGGACTAGGGGCGAACGCTGCGACCTGGAAGACAGAGACCGCTCCGAACTG
  TCTCCCCTGCTGCTGTCTACAACACAATGGCAAGTCTTGCCCTGCTCATTTACTACCCTGCCCGCACTGT
  CCACTGGGCTGATTCACCTGCACCAGAACATTGTGGATGTTCAGTACTTGTATGGCGTCGGCTCCTCTAT
  TGCCAGTTGGGCTATTAAGTGGGAATATGTAGTGCTCCTGTTCTTGCTCCTCGCAGACGCCCGAGTGTGC
  TCATGCCTGTGGATGATGCTGCTGATCTCCCAAGCCGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102217 representing NP_751921
  Red=Cloning sites Green=Tags
  
  METHVTGGSAGRTTAGLVGLLTPGAKQNIQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFNSSGC
  PERLASCRRLTDFAQGWGPISYANGSGLDERPYCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRS
  GAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGNNTLLCPTDCFRKHPEA
  TYSRCGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACNWTRGERCDLEDRDRSEL
  SPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSSIASWAIKWEYVVLLFLLLADARVC
  SCLWMMLLISQAEA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004102
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004102.1, NP_751921
• RefSeq ORF: 1092
• MW: 40.2 kDa