ha (NC_004908) Virus Tagged ORF Clone

CAT#: VC102429

Myc-DDK-tagged ORF clone of viral ORF for Hemagglutinin [Influenza A virus (A/Hong Kong/1073/99(H9N2))], codon optimized for human cell expression, NP_859037

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102429 represents NCBI reference of NP_859037 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAATCTCCCTTATTACAATCCTGCTGGTGGTTACCGCTAGCAACGCTGATAAAATTTGCATTG
  GCCACCAGAGCACCAATAGTACCGAAACTGTTGATACACTGACTGAAACAAACGTGCCAGTGACACATGC
  CAAAGAATTGCTCCACACAGAACACAACGGCATGCTTTGCGCAACATCCCTTGGGCACCCCTTGATATTG
  GACACATGCACGATCGAGGGCCTCGTGTATGGGAATCCTAGCTGCGACCTGCTGCTCGGAGGTAGGGAGT
  GGTCCTATATTGTTGAACGGAGTTCCGCGGTCAACGGGACGTGTTATCCGGGAAATGTAGAAAACCTCGA
  AGAACTGCGAACACTGTTCAGTTCCGCGTCTAGCTATCAGCGGATCCAGATCTTCCCAGACACCACCTGG
  AACGTCACGTATACGGGGACAAGCCGGGCATGCAGCGGAAGTTTCTACCGGTCCATGCGGTGGCTCACTC
  AAAAAAGCGGATTCTACCCTGTGCAGGACGCACAGTACACGAATAACAGGGGGAAGTCAATTCTCTTTGT
  ATGGGGCATCCATCATCCGCCAACATATACAGAGCAGACAAATCTGTATATCCGGAATGACACCACCACA
  TCCGTAACCACAGAGGATCTGAATCGCACGTTCAAGCCAGTAATCGGCCCTCGGCCCCTGGTGAATGGCT
  TGCAGGGGCGCATCGATTATTACTGGTCTGTCCTGAAGCCTGGGCAGACCTTGAGGGTGCGCAGCAATGG
  AAATCTTATTGCCCCGTGGTATGGGCATGTGCTCTCTGGCGGAAGCCATGGAAGGATCCTGAAGACCGAC
  CTTAAGGGAGGCAACTGTGTCGTACAGTGCCAGACCGAGAAAGGAGGACTTAACTCTACTCTTCCTTTCC
  ACAATATTAGTAAGTACGCCTTTGGCACCTGCCCAAAGTATGTGAGAGTGAACAGCCTGAAACTGGCCGT
  GGGTCTGCGGAACGTTCCAGCCCGCAGCTCTCGCGGCCTGTTCGGCGCCATAGCCGGATTCATTGAAGGC
  GGTTGGCCCGGACTGGTGGCCGGATGGTACGGCTTCCAGCATTCTAACGATCAGGGGGTCGGCATGGCGG
  CTGACAGGGACAGCACACAGAAGGCAATCGATAAAATTACATCCAAGGTCAATAATATTGTCGACAAAAT
  GAACAAACAGTACGAAATCATCGACCACGAATTCTCCGAAGTTGAGACTAGACTCAATATGATCAACAAC
  AAGATTGACGACCAAATTCAGGACGTATGGGCGTACAACGCCGAACTCCTGGTACTGCTGGAAAACCAGA
  AAACCCTTGATGAACACGATGCGAACGTCAATAACCTGTATAACAAAGTTAAACGGGCACTCGGTAGCAA
  CGCAATGGAAGACGGCAAGGGCTGCTTCGAGCTGTATCACAAGTGTGATGACCAGTGCATGGAGACTATT
  AGAAATGGTACCTATAATCGCAGGAAATATAGGGAGGAGAGCCGACTTGAAAGACAAAAAATAGAAGGAG
  TGAAACTGGAGAGTGAGGGTACCTACAAGATCCTCACCATTTACTCCACTGTAGCCAGCTCCCTCGTGCT
  CGCGATGGGCTTCGCCGCATTCCTGTTTTGGGCTATGTCCAACGGAAGCTGTCGGTGCAACATTTGTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102429 representing NP_859037
  Red=Cloning sites Green=Tags
  
  METISLITILLVVTASNADKICIGHQSTNSTETVDTLTETNVPVTHAKELLHTEHNGMLCATSLGHPLIL
  DTCTIEGLVYGNPSCDLLLGGREWSYIVERSSAVNGTCYPGNVENLEELRTLFSSASSYQRIQIFPDTTW
  NVTYTGTSRACSGSFYRSMRWLTQKSGFYPVQDAQYTNNRGKSILFVWGIHHPPTYTEQTNLYIRNDTTT
  SVTTEDLNRTFKPVIGPRPLVNGLQGRIDYYWSVLKPGQTLRVRSNGNLIAPWYGHVLSGGSHGRILKTD
  LKGGNCVVQCQTEKGGLNSTLPFHNISKYAFGTCPKYVRVNSLKLAVGLRNVPARSSRGLFGAIAGFIEG
  GWPGLVAGWYGFQHSNDQGVGMAADRDSTQKAIDKITSKVNNIVDKMNKQYEIIDHEFSEVETRLNMINN
  KIDDQIQDVWAYNAELLVLLENQKTLDEHDANVNNLYNKVKRALGSNAMEDGKGCFELYHKCDDQCMETI
  RNGTYNRRKYREESRLERQKIEGVKLESEGTYKILTIYSTVASSLVLAMGFAAFLFWAMSNGSCRCNICI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004908
• ORF Size: 1680 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004908.1, NP_859037
• RefSeq ORF: 1680
• Locus ID: 1460996
• MW: 62.5 kDa