HA1 (NC_004908) Virus Tagged ORF Clone

CAT#: VC102430

Myc-DDK-tagged ORF clone of viral ORF for HA1 [Influenza A virus (A/Hong Kong/1073/99(H9N2))], codon optimized for human cell expression, YP_529488

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102430 represents NCBI reference of YP_529488 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAGATTTGTATCGGACATCAGAGTACTAACAGTACAGAAACTGTGGATACCCTCACAGAGACCA
  ATGTGCCAGTCACACACGCCAAGGAGCTCCTCCACACAGAACACAACGGAATGCTTTGTGCTACTTCCCT
  GGGGCATCCCTTGATCCTGGATACCTGTACAATTGAAGGGCTTGTATATGGAAACCCCAGCTGCGACCTG
  CTGTTGGGAGGCAGGGAATGGAGTTATATCGTAGAGCGCAGCTCCGCCGTCAACGGAACATGTTATCCAG
  GCAACGTGGAGAATCTCGAAGAGCTGCGCACATTGTTTTCATCAGCCAGTTCATATCAGAGGATTCAAAT
  TTTCCCGGACACTACCTGGAACGTGACTTACACCGGGACGTCCAGGGCCTGCTCCGGCTCCTTTTACCGC
  AGTATGCGATGGTTGACCCAGAAGTCTGGTTTTTATCCTGTGCAAGATGCGCAGTATACCAATAATAGAG
  GAAAGTCTATACTCTTTGTGTGGGGAATCCACCATCCTCCGACGTACACAGAACAGACAAACCTGTACAT
  CAGGAACGACACTACCACATCAGTGACAACTGAAGACCTTAACCGGACCTTCAAACCTGTCATTGGACCC
  AGACCTCTGGTGAATGGTCTGCAAGGCAGGATAGATTACTATTGGTCAGTTCTGAAACCAGGCCAGACTC
  TTAGGGTGCGCTCTAATGGAAATCTCATTGCTCCATGGTATGGCCACGTCCTCAGTGGAGGGAGCCACGG
  CCGGATTCTGAAGACAGATCTCAAAGGTGGCAACTGCGTAGTGCAGTGTCAAACTGAGAAGGGAGGCCTG
  AACTCTACATTGCCATTTCATAACATCAGTAAGTACGCATTTGGAACTTGCCCCAAGTACGTGAGAGTAA
  ATTCTTTGAAACTGGCGGTTGGCCTCCGCAATGTCCCCGCAAGGTCATCACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102430 representing YP_529488
  Red=Cloning sites Green=Tags
  
  MDKICIGHQSTNSTETVDTLTETNVPVTHAKELLHTEHNGMLCATSLGHPLILDTCTIEGLVYGNPSCDL
  LLGGREWSYIVERSSAVNGTCYPGNVENLEELRTLFSSASSYQRIQIFPDTTWNVTYTGTSRACSGSFYR
  SMRWLTQKSGFYPVQDAQYTNNRGKSILFVWGIHHPPTYTEQTNLYIRNDTTTSVTTEDLNRTFKPVIGP
  RPLVNGLQGRIDYYWSVLKPGQTLRVRSNGNLIAPWYGHVLSGGSHGRILKTDLKGGNCVVQCQTEKGGL
  NSTLPFHNISKYAFGTCPKYVRVNSLKLAVGLRNVPARSSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004908
• ORF Size: 963 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004908.1, YP_529488
• RefSeq ORF: 963
• MW: 35.7 kDa