HA1 (NC_007374) Virus Tagged ORF Clone

CAT#: VC102448

Myc-DDK-tagged ORF clone of viral ORF for HA1 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308873

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102448 represents NCBI reference of YP_308873 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAAATCTGCATCGGGTACCACGCCAACAATAGCACAGAGAAGGTCGATACCATTCTGGAAAGGA
  ACGTTACCGTTACCCATGCCAAAGACATCCTCGAGAAGACTCATAACGGCAAGCTGTGCAAGCTGAACGG
  CATTCCCCCGCTCGAATTGGGCGACTGCTCCATTGCCGGCTGGCTCCTGGGTAACCCCGAATGCGACAGG
  CTCCTCTCTGTGCCAGAATGGTCTTACATCATGGAAAAAGAGAATCCTCGGTACTCTCTGTGCTATCCCG
  GCTCATTTAACGACTATGAGGAGCTCAAGCATCTGCTGAGTTCCGTAAAGCACTTTGAAAAAGTGAAAAT
  TCTCCCCAAGGATCGGTGGACACAGCATACCACCACTGGAGGCTCATGGGCCTGTGCCGTGAGTGGTAAA
  CCCTCTTTTTTCAGAAACATGGTGTGGTTGACGCGGAAAGGGTCAAATTACCCCGTGGCCAAGGGTAGCT
  ACAACAACACATCTGGTGAGCAGATGCTGATCATTTGGGGCGTCCATCACCCAAACGACGAAGCAGAGCA
  AAGGGCACTGTATCAAAATGTTGGGACATACGTTTCCGTTGCTACCTCTACACTTTACAAGCGGAGTATT
  CCAGAGATTGCGGCCAGGCCCAAAGTGAACGGCCTGGGTCGGAGAATGGAATTTTCCTGGACACTTCTGG
  ACATGTGGGACACCATTAATTTCGAGTCCACTGGGAATCTTGTGGCCCCCGAATATGGGTTCAAGATCTC
  TAAAAGAGGCAGTTCTGGGATTATGAAGACAGAGGGCACCTTGGAGAATTGTGAGACAAAGTGCCAGACC
  CCCCTTGGTGCAATTAATACCACTCTGCCTTTCCATAATGTTCACCCTCTGACAATCGGAGAGTGCCCTA
  AGTACGTTAAGTCAGAAAAGCTCGTCCTCGCAACCGGTCTGCGAAACGTGCCCCAGATCGAATCCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102448 representing YP_308873
  Red=Cloning sites Green=Tags
  
  MDQICIGYHANNSTEKVDTILERNVTVTHAKDILEKTHNGKLCKLNGIPPLELGDCSIAGWLLGNPECDR
  LLSVPEWSYIMEKENPRYSLCYPGSFNDYEELKHLLSSVKHFEKVKILPKDRWTQHTTTGGSWACAVSGK
  PSFFRNMVWLTRKGSNYPVAKGSYNNTSGEQMLIIWGVHHPNDEAEQRALYQNVGTYVSVATSTLYKRSI
  PEIAARPKVNGLGRRMEFSWTLLDMWDTINFESTGNLVAPEYGFKISKRGSSGIMKTEGTLENCETKCQT
  PLGAINTTLPFHNVHPLTIGECPKYVKSEKLVLATGLRNVPQIESR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007374
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007374.1, YP_308873
• RefSeq ORF: 978
• MW: 36.6 kDa