HA2 (NC_007374) Virus Tagged ORF Clone

CAT#: VC102449

Myc-DDK-tagged ORF clone of viral ORF for HA2 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308874

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102449 represents NCBI reference of YP_308874 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCTTCGGAGCAATTGCAGGCTTCATTGAAGGAGGCTGGCAAGGTATGGTCGACGGCTGGTACG
  GATATCATCACTCTAATGACCAGGGATCTGGTTACGCGGCGGACAAAGAAAGCACACAGAAAGCGTTCAA
  CGGGATCACTAATAAGGTCAACAGTGTGATCGAGAAGATGAATACTCAGTTTGAGGCAGTCGGAAAGGAA
  TTCTCCAACCTGGAAAAAAGATTGGAGAATCTGAACAAAAAGATGGAAGATGGGTTTCTGGATGTGTGGA
  CCTACAATGCCGAACTGCTGGTGCTGATGGAGAATGAGAGGACTCTGGACTTCCATGATTCTAACGTCAA
  AAACCTGTACGACAAGGTTCGCATGCAGCTCAGGGATAACGTAAAGGAGCTCGGGAATGGATGTTTTGAG
  TTTTACCATAAATGCGACAACGAGTGCATGGATTCTGTTAAAAACGGCACGTATGATTATCCTAAATATG
  AAGAGGAGAGCAAGCTGAACCGGAATGAGATTAAGGGAGTGAAGCTGAGTTCAATGGGCGTTTATCAAAT
  ACTCGCTATATACGCTACAGTTGCAGGGAGCCTGAGTCTGGCCATTATGATGGCGGGCATATCCTTCTGG
  ATGTGTTCTAATGGTTCCCTGCAGTGCAGGATATGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102449 representing YP_308874
  Red=Cloning sites Green=Tags
  
  MGLFGAIAGFIEGGWQGMVDGWYGYHHSNDQGSGYAADKESTQKAFNGITNKVNSVIEKMNTQFEAVGKE
  FSNLEKRLENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRMQLRDNVKELGNGCFE
  FYHKCDNECMDSVKNGTYDYPKYEEESKLNRNEIKGVKLSSMGVYQILAIYATVAGSLSLAIMMAGISFW
  MCSNGSLQCRICI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007374
• ORF Size: 669 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007374.1, YP_308874
• RefSeq ORF: 669
• MW: 25.3 kDa