HA (NC_007366) Virus Tagged ORF Clone

CAT#: VC102455

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308839


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Reconstitution Protocol

USD 720.00

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol HA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102455 represents NCBI reference of YP_308839 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACCATCATTGCCCTGAGCTATATCTTGTGTCTCGTTTTCGCGCAGAAGCTTCCGGGAAACGACA
ATTCTACAGCAACTCTGTGTCTCGGACACCATGCGGTGCCTAACGGAACAATTGTGAAGACCATAACGAA
CGACCAGATCGAAGTGACAAACGCTACAGAGCTCGTGCAATCTTCCTCCACAGGGGGGATCTGTGACTCA
CCACATCAAATCCTGGACGGCGAAAACTGCACCCTGATAGACGCCCTGCTGGGTGATCCCCAGTGTGACG
GGTTCCAGAACAAGAAGTGGGACCTTTTTGTGGAACGGTCTAAGGCATACAGTAATTGCTATCCTTATGA
CGTCCCCGACTACGCATCCCTCAGGTCACTCGTAGCTAGTTCTGGTACACTGGAGTTCAATAATGAGAGC
TTCAACTGGACGGGCGTTACCCAAAACGGAACTTCCAGCGCTTGTAAGCGCCGGTCCAATAATAGCTTTT
TTTCCAGGCTGAACTGGCTGACTCATCTGAAATTTAAGTACCCTGCCCTTAATGTGACCATGCCCAACAA
CGAGAAGTTCGATAAACTCTATATTTGGGGCGTCCACCATCCAGGGACCGATAACGACCAAATTTCCCTG
TACGCCCAGGCTTCTGGCCGCATCACAGTATCCACGAAAAGGTCCCAGCAGACAGTTATCCCTTCTATCG
GCTCCCGCCCACGCATCCGAGACGTGCCTTCCAGGATCTCCATATATTGGACGATCGTCAAGCCCGGAGA
CATTCTCCTGATTAACTCAACTGGCAATCTCATTGCTCCTAGAGGCTACTTTAAAATCCGGTCCGGCAAA
TCATCCATCATGCGATCCGACGCCCCAATTGGAAAGTGCAACAGTGAGTGTATCACTCCAAACGGCAGCA
TCCCTAACGATAAACCCTTTCAGAATGTCAACCGAATCACATACGGAGCTTGCCCACGGTACGTCAAGCA
GAACACCCTTAAACTGGCCACCGGGATGAGAAACGTCCCGGAAAAACAGACCAGAGGCATCTTCGGCGCT
ATAGCGGGGTTTATTGAGAACGGGTGGGAAGGTATGGTGGACGGCTGGTATGGCTTCAGACATCAAAATA
GTGAGGGTACAGGACAGGCCGCCGACCTGAAGTCCACTCAGGCTGCAATTAATCAGATTAATGGCAAGCT
CAATCGGTTGATCGGGAAGACTAATGAGAAGTTTCACCAAATCGAGAAGGAATTCTCAGAAGTGGAAGGT
CGGATCCAGGATCTCGAGAAGTACGTTGAAGATACCAAAATTGATCTGTGGTCCTATAACGCGGAGCTGC
TGGTGGCCCTTGAGAATCAACATACAATCGATTTGACTGACAGCGAGATGAATAAACTGTTTGAACGCAC
AAAGAAGCAGTTGAGAGAGAACGCCGAAGATATGGGCAATGGCTGTTTCAAGATCTATCACAAGTGTGAT
AACGCTTGCATCGGATCAATTAGAAACGGGACATACGACCATGATGTCTATAGAGATGAGGCACTGAACA
ATCGGTTTCAAATCAAGGGCGTCGAACTTAAGTCCGGCTATAAAGACTGGATCTTGTGGATAAGCTTTGC
TATTTCTTGTTTTTTGCTGTGTGTGGCACTGCTCGGTTTCATCATGTGGGCATGCCAGAAAGGCAATATC
CGCTGTAACATCTGCATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102455 representing YP_308839
Red=Cloning sites Green=Tags

MKTIIALSYILCLVFAQKLPGNDNSTATLCLGHHAVPNGTIVKTITNDQIEVTNATELVQSSSTGGICDS
PHQILDGENCTLIDALLGDPQCDGFQNKKWDLFVERSKAYSNCYPYDVPDYASLRSLVASSGTLEFNNES
FNWTGVTQNGTSSACKRRSNNSFFSRLNWLTHLKFKYPALNVTMPNNEKFDKLYIWGVHHPGTDNDQISL
YAQASGRITVSTKRSQQTVIPSIGSRPRIRDVPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGK
SSIMRSDAPIGKCNSECITPNGSIPNDKPFQNVNRITYGACPRYVKQNTLKLATGMRNVPEKQTRGIFGA
IAGFIENGWEGMVDGWYGFRHQNSEGTGQAADLKSTQAAINQINGKLNRLIGKTNEKFHQIEKEFSEVEG
RIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFERTKKQLRENAEDMGNGCFKIYHKCD
NACIGSIRNGTYDHDVYRDEALNNRFQIKGVELKSGYKDWILWISFAISCFLLCVALLGFIMWACQKGNI
RCNICI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007366
ORF Size 1698 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_007366.1, YP_308839
RefSeq ORF 1698
Locus ID 3655151
MW 63.5 kDa

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