HA1 (NC_007366) Virus Tagged ORF Clone

CAT#: VC102456

Myc-DDK-tagged ORF clone of viral ORF for HA1 [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308875

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102456 represents NCBI reference of YP_308875 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGCTCCCAGGAAACGACAACAGTACCGCCACTTTGTGCCTCGGGCACCACGCAGTGCCCAATG
  GGACAATAGTGAAAACAATCACTAATGACCAGATTGAGGTGACGAATGCCACTGAACTGGTGCAGTCTAG
  TTCCACTGGCGGGATCTGTGACAGCCCTCATCAGATTCTGGATGGAGAGAACTGTACACTGATAGATGCT
  TTGCTTGGAGACCCCCAATGTGACGGATTTCAAAACAAGAAGTGGGACCTGTTTGTGGAGCGGAGCAAAG
  CTTACTCAAACTGTTACCCCTATGACGTTCCTGATTACGCCAGTCTGAGGTCCCTCGTGGCTTCTAGCGG
  CACTCTGGAGTTTAACAATGAATCCTTTAACTGGACCGGCGTCACACAGAATGGGACCAGTTCTGCATGC
  AAGAGAAGATCCAACAACTCCTTTTTCTCCAGACTCAACTGGTTGACGCACCTCAAATTCAAGTATCCGG
  CTCTCAATGTCACCATGCCTAACAATGAAAAGTTCGACAAGCTTTATATCTGGGGCGTACACCACCCGGG
  CACGGATAATGACCAAATCTCATTGTACGCTCAGGCCTCCGGACGGATCACTGTGAGCACGAAGAGATCT
  CAGCAGACCGTAATACCCTCCATCGGTTCACGCCCAAGGATAAGAGATGTGCCCTCCCGAATCTCTATTT
  ATTGGACCATCGTCAAGCCCGGCGATATTCTGCTCATCAACTCTACAGGAAACCTCATCGCACCTAGAGG
  CTACTTCAAGATTCGCAGCGGAAAAAGCAGCATCATGCGGTCCGACGCGCCAATTGGTAAATGCAACAGC
  GAGTGCATAACCCCCAACGGGAGTATACCGAATGATAAGCCGTTCCAGAATGTTAATAGAATAACTTACG
  GCGCCTGCCCGCGGTATGTGAAGCAAAATACGCTTAAACTCGCGACAGGAATGAGGAACGTGCCAGAGAA
  ACAGACCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102456 representing YP_308875
  Red=Cloning sites Green=Tags
  
  MQKLPGNDNSTATLCLGHHAVPNGTIVKTITNDQIEVTNATELVQSSSTGGICDSPHQILDGENCTLIDA
  LLGDPQCDGFQNKKWDLFVERSKAYSNCYPYDVPDYASLRSLVASSGTLEFNNESFNWTGVTQNGTSSAC
  KRRSNNSFFSRLNWLTHLKFKYPALNVTMPNNEKFDKLYIWGVHHPGTDNDQISLYAQASGRITVSTKRS
  QQTVIPSIGSRPRIRDVPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGKSSIMRSDAPIGKCNS
  ECITPNGSIPNDKPFQNVNRITYGACPRYVKQNTLKLATGMRNVPEKQTR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007366
• ORF Size: 990 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007366.1, YP_308875
• RefSeq ORF: 990
• MW: 36.6 kDa