HA (NC_002017) Virus Tagged ORF Clone

CAT#: VC102475

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for haemagglutinin [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, NP_040980


  View other clones from "Virus" (2)

Reconstitution Protocol

USD 720.00

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol HA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102475 represents NCBI reference of NP_040980 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCAAATCTGTTGGTTCTGCTCTGCGCACTTGCGGCGGCGGACGCAGACACAATTTGCATTGGAT
ACCACGCCAACAATAGTACAGATACAGTAGACACCGTTCTGGAAAAGAATGTGACAGTCACACATAGCGT
AAATCTCTTGGAGGATTCCCATAATGGGAAGCTGTGCAGGCTGAAAGGAATCGCGCCCCTGCAACTCGGA
AAATGCAATATTGCCGGGTGGCTCCTGGGAAATCCCGAGTGCGACCCGCTCCTTCCCGTGAGGTCCTGGA
GCTATATAGTTGAGACACCAAACAGTGAGAACGGCATCTGTTACCCCGGGGATTTTATTGACTACGAGGA
GCTGCGGGAGCAACTGAGTAGTGTCTCCTCCTTCGAGCGGTTCGAGATTTTCCCCAAGGAATCATCTTGG
CCGAATCATAACACAACCAAAGGTGTCACTGCCGCTTGTTCCCATGCTGGAAAGTCTTCCTTTTATCGGA
ACTTGTTGTGGCTCACCGAAAAAGAGGGCTCCTATCCTAAGCTGAAGAATTCATATGTGAATAAAAAAGG
GAAGGAGGTCCTGGTGTTGTGGGGGATTCATCACCCAAGCAACTCTAAGGACCAGCAAAACATATACCAG
AACGAGAACGCTTACGTTAGCGTGGTGACCAGCAACTACAACAGAAGGTTCACACCAGAAATCGCTGAGA
GGCCAAAGGTACGCGACCAGGCGGGGAGAATGAATTATTATTGGACCCTGCTCAAACCCGGCGACACTAT
CATATTTGAGGCTAATGGCAACCTCATTGCTCCAAGATATGCGTTTGCATTGTCACGGGGGTTCGGGTCT
GGAATAATTACTAGTAATGCGAGTATGCATGAGTGTAACACGAAGTGCCAGACACCGCTGGGCGCGATCA
ATTCCTCCCTCCCTTTCCAGAACATTCATCCAGTCACCATCGGAGAGTGCCCCAAGTATGTACGGAGTGC
AAAACTTCGGATGGTGACAGGTCTTCGCAACATACCATCTATCCAGTCCAGAGGACTCTTCGGCGCCATC
GCAGGATTTATAGAGGGGGGCTGGACCGGAATGATCGATGGATGGTACGGTTATCATCACCAGAATGAAC
AGGGAAGCGGCTATGCCGCCGACCAGAAGTCCACACAAAACGCAATCAACGGTATCACCAATAAAGTCAA
CAGTGTAATCGAGAAGATGAACATTCAATTCACGGCCGTTGGGAAGGAGTTTAACAAGCTGGAGAAGAGG
ATGGAAAATCTGAATAAGAAAGTGGATGACGGATTTCTGGATATTTGGACCTACAACGCTGAGCTGCTCG
TGCTCCTGGAAAATGAGAGGACACTTGACTTTCACGACTCTAACGTCAAAAACCTGTACGAAAAAGTGAA
GAGTCAGCTGAAAAACAACGCTAAGGAGATTGGGAACGGCTGTTTCGAGTTCTATCACAAATGTGACAAT
GAGTGCATGGAAAGCGTCCGCAATGGGACATACGACTATCCAAAATATAGCGAGGAATCAAAACTGAATA
GAGAGAAGGTAGACGGGGTAAAGTTGGAGTCCATGGGTATTTACCAGATACTCGCCATTTACAGTACAGT
GGCATCATCACTGGTGCTCCTGGTCTCTTTGGGAGCAATTTCTTTTTGGATGTGTAGCAATGGGAGCCTG
CAATGCAGAATTTGTATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102475 representing NP_040980
Red=Cloning sites Green=Tags

MKANLLVLLCALAAADADTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLG
KCNIAGWLLGNPECDPLLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSW
PNHNTTKGVTAACSHAGKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPSNSKDQQNIYQ
NENAYVSVVTSNYNRRFTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPRYAFALSRGFGS
GIITSNASMHECNTKCQTPLGAINSSLPFQNIHPVTIGECPKYVRSAKLRMVTGLRNIPSIQSRGLFGAI
AGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNSVIEKMNIQFTAVGKEFNKLEKR
MENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCDN
ECMESVRNGTYDYPKYSEESKLNREKVDGVKLESMGIYQILAIYSTVASSLVLLVSLGAISFWMCSNGSL
QCRICI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_002017
ORF Size 1698 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_002017.1, NP_040980
RefSeq ORF 1698
Locus ID 956529
MW 63.4 kDa

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