HA1 (NC_002017) Virus Tagged ORF Clone

CAT#: VC102476

Myc-DDK-tagged ORF clone of viral ORF for HA1 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, YP_163735

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102476 represents NCBI reference of YP_163735 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCATTTGCATCGGATACCACGCTAACAATAGCACCGATACCGTGGACACCGTGCTGGAGAAAA
  ACGTGACCGTGACACACAGCGTCAATCTCCTCGAAGACTCCCATAACGGCAAGCTCTGTCGGCTGAAAGG
  CATCGCCCCACTGCAGCTGGGAAAATGCAACATCGCCGGCTGGCTGCTCGGCAACCCCGAGTGCGACCCC
  CTGCTGCCCGTCCGGTCCTGGAGCTACATCGTCGAGACCCCCAACAGCGAGAACGGCATCTGCTACCCCG
  GCGACTTCATCGACTACGAGGAGCTGCGGGAGCAGCTCAGCAGCGTGAGCAGCTTCGAGCGGTTCGAGAT
  CTTCCCCAAGGAGAGCAGCTGGCCCAACCACAACACAACCAAGGGCGTGACCGCCGCCTGCTCCCACGCC
  GGCAAGAGCAGCTTCTACCGGAACCTGCTGTGGCTGACCGAGAAGGAGGGCAGCTATCCCAAGCTCAAAA
  ACAGCTACGTGAACAAGAAGGGCAAGGAGGTCCTGGTCCTGTGGGGCATCCACCACCCCAGCAACAGCAA
  GGACCAGCAGAACATCTACCAAAACGAGAACGCCTACGTGTCCGTGGTGACTAGCAATTACAACCGGCGG
  TTCACCCCCGAGATCGCCGAGCGGCCCAAAGTGCGGGACCAGGCAGGCCGGATGAACTACTACTGGACTC
  TGCTCAAGCCCGGCGACACCATCATCTTCGAGGCCAACGGGAACCTGATCGCCCCCCGGTACGCCTTTGC
  CCTGAGCCGGGGCTTCGGCAGCGGCATCATCACCTCCAACGCCAGCATGCACGAATGCAACACCAAGTGC
  CAGACCCCCCTGGGCGCAATCAACAGCAGCCTGCCCTTCCAGAATATCCACCCTGTGACTATCGGCGAGT
  GCCCCAAGTACGTGCGGAGCGCTAAGCTGAGAATGGTCACTGGCCTGCGGAATATTCCATCCATCCAGAG
  CCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102476 representing YP_163735
  Red=Cloning sites Green=Tags
  
  MDTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWLLGNPECDP
  LLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTTKGVTAACSHA
  GKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPSNSKDQQNIYQNENAYVSVVTSNYNRR
  FTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPRYAFALSRGFGSGIITSNASMHECNTKC
  QTPLGAINSSLPFQNIHPVTIGECPKYVRSAKLRMVTGLRNIPSIQSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002017
• ORF Size: 984 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002017.1, YP_163735
• RefSeq ORF: 984
• MW: 36.8 kDa