NB (NC_002209) Virus Tagged ORF Clone

CAT#: VC102484

Myc-DDK-tagged ORF clone of viral ORF for NB protein [Influenza B virus], codon optimized for human cell expression, NP_056662

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102484 represents NCBI reference of NP_056662 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACGCAACTTTTAATTGCACCAACATTAACCCTATCACTCATATACGAGGGTCCATCATCATTA
  CTATCTGCGTGTCCCTCATTGTTATCCTCATAGTTTTTGGATGTATCGCCAAGATATTTATCAATAAGAA
  CAATTGTACTAACAACGTCATCCGGGTCCACAAAAGGATTAAATGCCCCGATTGTGAGCCTTTCTGTAAC
  AAGCGCGATGATATTAGCACTCCCAGAGCAGGGGTGGATATTCCAAGCTTCATCCTTCCAGGCTTGAACC
  TGAGTGAAGGGACGCCCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102484 representing NP_056662
  Red=Cloning sites Green=Tags
  
  MNNATFNCTNINPITHIRGSIIITICVSLIVILIVFGCIAKIFINKNNCTNNVIRVHKRIKCPDCEPFCN
  KRDDISTPRAGVDIPSFILPGLNLSEGTPN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002209
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002209.1, NP_056662
• RefSeq ORF: 300
• Locus ID: 956540
• MW: 11.0 kDa