NA (NC_002209) Virus Tagged ORF Clone

CAT#: VC102485

Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza B virus], codon optimized for human cell expression, NP_056663

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102485 represents NCBI reference of NP_056663 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCTCCACCGTGCAGACCCTCACCCTGTTGCTTACTAGCGGAGGGGTGCTTCTCTCTCTGTATG
  TTTCAGCGTCTCTGTCATATCTTCTTTATTCAGATGTGCTGCTCAAGTTTTCCTCCACAAAAACTACTGC
  CCCGACTATGAGCCTCGAATGCACCAATGCCAGCAACGCCCAGACAGTGAATCATTCCGCCACTAAGGAG
  ATGACCTTTCCTCCCCCCGAGCCAGAGTGGACTTACCCACGGCTTTCTTGCCAGGGAAGCACTTTTCAGA
  AGGCACTGCTTATCTCTCCCCACCGGTTTGGCGAGATCAAGGGCAACAGCGCACCTCTGATTATTCGAGA
  ACCTTTCGTTGCGTGTGGCCCTAAAGAGTGCAGGCATTTCGCTCTGACTCATTACGCAGCACAACCGGGA
  GGATATTACAATGGAACTCGCAAGGACCGCAACAAACTCCGCCACCTGGTAAGCGTCAAGCTGGGTAAAA
  TTCCTACGGTTGAAAACTCTATCTTCCATATGGCCGCGTGGTCTGGGTCTGCCTGCCACGATGGGAGAGA
  GTGGACCTATATAGGGGTAGATGGCCCTGACAACGACGCACTCGTAAAGATTAAATATGGAGAGGCATAC
  ACCGACACATACCACAGCTACGCACATAACATACTGAGGACTCAGGAGAGCGCCTGCAATTGCATTGGCG
  GCGATTGTTATCTGATGATCACCGATGGCTCCGCGTCCGGTATCTCAAAGTGCAGGTTCCTTAAGATTAG
  AGAGGGACGAATAATCAAGGAGATCCTCCCAACGGGACGGGTTGAACACACGGAAGAGTGCACATGCGGA
  TTCGCAAGTAACAAGACAATTGAGTGTGCGTGTCGGGACAATTCCTACACGGCGAAACGCCCCTTTGTGA
  AACTTAATGTCGAAACCGATACTGCCGAAATTCGACTCATGTGCACGAAAACCTATCTTGACACACCCAG
  ACCAGACGACGGTAGTATCGCCGGTCCTTGCGAGAGCAATGGCGATAAATGGCTCGGGGGAATTAAGGGA
  GGATTCGTTCATCAGAGGATGGCATCTAAAATCGGTCGCTGGTACTCACGCACAATGAGCAAAACTAACA
  GGATGGGCATGGAGCTCTATGTGAAGTATGACGGGGACCCCTGGACCGACAGCGATGCTCTGACATTGAG
  CGGGGTGATGGTATCTATCGAAGAGCCCGGTTGGTACTCCTTCGGATTTGAGATTAAGGACAAAAAATGC
  GATGTCCCTTGCATTGGTATAGAGATGGTACATGACGGAGGCAAAGATACATGGCATTCCGCCGCCACCG
  CAATCTATTGCCTGATGGGTTCTGGCCAGCTCCTTTGGGATACCGTGACTGGAGTGGATATGGCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102485 representing NP_056663
  Red=Cloning sites Green=Tags
  
  MLPSTVQTLTLLLTSGGVLLSLYVSASLSYLLYSDVLLKFSSTKTTAPTMSLECTNASNAQTVNHSATKE
  MTFPPPEPEWTYPRLSCQGSTFQKALLISPHRFGEIKGNSAPLIIREPFVACGPKECRHFALTHYAAQPG
  GYYNGTRKDRNKLRHLVSVKLGKIPTVENSIFHMAAWSGSACHDGREWTYIGVDGPDNDALVKIKYGEAY
  TDTYHSYAHNILRTQESACNCIGGDCYLMITDGSASGISKCRFLKIREGRIIKEILPTGRVEHTEECTCG
  FASNKTIECACRDNSYTAKRPFVKLNVETDTAEIRLMCTKTYLDTPRPDDGSIAGPCESNGDKWLGGIKG
  GFVHQRMASKIGRWYSRTMSKTNRMGMELYVKYDGDPWTDSDALTLSGVMVSIEEPGWYSFGFEIKDKKC
  DVPCIGIEMVHDGGKDTWHSAATAIYCLMGSGQLLWDTVTGVDMAL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002209
• ORF Size: 1398 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_002209.1, NP_056663
• RefSeq ORF: 1398
• Locus ID: 956541
• MW: 51.4 kDa