GRK5 (NM_005308) Human Mutant ORF Clone

CAT#: RC402763

GRK5 Mutant (Q41L), Myc-DDK-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase 5 (GRK5) as transfection-ready DNA

Specifications

Product Data

• Mutation Description: Q41L
• Affected Codon#: 41
• Affected NT#: 122
• Tag: Myc-DDK
• Effect: Decreased mortality in heart failure, association with
• Symbol: GRK5
• Synonyms: FP2025; GPRK5
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_005308
• ORF Size: 1770 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC402763 representing NM_005308
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGAGGAAAGCGCA
  AAGGGAAAAGCAAGAAGTGGAAAGAAATCCTGAAGTTCCCTCACATTAGCCTGTGTGAAGACCTCCGAAG
  GACCATAGACAGAGATTACTGCAGTTTATGTGACAAGCAGCCAATCGGGAGGCTGCTTTTCCGGCAGTTT
  TGTGAAACCAGGCCTGGGCTGGAGTGTTACATTCAGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTC
  CAGATGAAAAACTGGGAGAGAAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCTGTTTT
  CATAGCCCAAGTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTACAGAAGCCGTGCAAAGAA
  CTCTTTTCTGCCTGTGCACAGTCTGTCCACGAGTACCTGAGGGGAGAACCATTCCACGAATATCTGGACA
  GCATGTTTTTTGACCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCGGTGACCAAAAACACTTTCAG
  GCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTCTGTGCCTGCCAGGTTCGGGCCACGGGTAAA
  ATGTATGCCTGCAAGCGCTTGGAGAAGAAGAGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCCTCAATG
  AGAAGCAGATCCTCGAGAAGGTCAACAGTCAGTTTGTGGTCAACCTGGCCTATGCCTACGAGACCAAGGA
  TGCACTGTGCTTGGTCCTGACCATCATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCAAC
  CCTGGCTTCGAGGAGGAGCGGGCCTTGTTTTATGCGGCAGAGATCCTCTGCGGCTTAGAAGACCTCCACC
  GTGAGAACACCGTCTACCGAGATCTGAAACCTGAAAACATCCTGTTAGATGATTATGGCCACATTAGGAT
  CTCAGACCTGGGCTTGGCTGTGAAGATCCCCGAGGGAGACCTGATCCGCGGCCGGGTGGGCACTGTTGGC
  TACATGGCTCCAGAGGTCCTGAACAACCAGAGGTACGGCCTGAGCCCCGACTACTGGGGCCTTGGCTGCC
  TCATCTATGAGATGATCGAGGGCCAGTCGCCGTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGT
  GGACCGCCGGGTCCTGGAGACGGAGGAGGTGTACTCCCACAAGTTCTCCGAGGAGGCCAAGTCCATCTGC
  AAGATGCTGCTCACGAAAGATGCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGGGCTGCAGAGGTCAAGA
  GACACCCCTTCTTCAGGAACATGAACTTCAAGCGCTTAGAAGCCGGGATGTTGGACCCTCCCTTCGTTCC
  AGACCCCCGCGCTGTGTACTGTAAGGACGTGCTGGACATCGAGCAGTTCTCCACTGTGAAGGGCGTCAAT
  CTGGACCACACAGACGACGACTTCTACTCCAAGTTCTCCACGGGCTCTGTGTCCATCCCATGGCAAAACG
  AGATGATAGAAACAGAATGCTTTAAGGAGCTGAACGTGTTTGGACCTAATGGTACCCTCCCGCCAGATCT
  GAACAGAAACCACCCTCCGGAACCGCCCAAGAAAGGGCTGCTCCAGAGACTCTTCAAGCGGCAGCATCAG
  AACAATTCCAAGAGTTCGCCCAGCTCCAAGACCAGTTTTAACCACCACATAAACTCAAACCATGTCAGCT
  CGAACTCCACCGGAAGCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC402763 representing NM_005308
  Red=Cloning site Green=Tags(s)
  
  MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISLCEDLRRTIDRDYCSLCDKQPIGRLLFRQF
  CETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKE
  LFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK
  MYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGN
  PGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVG
  YMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSIC
  KMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVN
  LDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQ
  NNSKSSPSSKTSFNHHINSNHVSSNSTGSS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NP_005299
• RefSeq Size: 1770 bp
• RefSeq ORF: 1773 bp
• Locus ID: 2869
• Cytogenetics: 10q26.11
• Domains: RGS, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 64.9 kDa
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]'