Human Alkaline Phosphatase (ALPP) activation kit by CRISPRa

CAT#: GA100174

ALPP CRISPRa kit - CRISPR gene activation of human alkaline phosphatase, placental

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ALPP
• Locus ID: 250
• Kit Components:

  GA100174G1, Alkaline Phosphatase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100174G2, Alkaline Phosphatase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100174G3, Alkaline Phosphatase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001632
• Synonyms: ALP; PALP; PLAP; PLAP-1
• Summary: 'The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Aug 2020]'