Human ASAH1 activation kit by CRISPRa

CAT#: GA100309

ASAH1 CRISPRa kit - CRISPR gene activation of human N-acylsphingosine amidohydrolase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ASAH1
• Locus ID: 427
• Kit Components:

  GA100309G1, ASAH1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100309G2, ASAH1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100309G3, ASAH1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001127505, NM_004315, NM_177924, NM_001363743
• Synonyms: AC; ACDase; ASAH; PHP; PHP32; SMAPME
• Summary: 'This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015]'