Human ATP5PF activation kit by CRISPRa

CAT#: GA100362

ATP5PF CRISPRa kit - CRISPR gene activation of human ATP synthase peripheral stalk subunit F6

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ATP5PF
• Locus ID: 522
• Kit Components:

  GA100362G1, ATP5PF gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100362G2, ATP5PF gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100362G3, ATP5PF gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001003696, NM_001003697, NM_001003701, NM_001003703, NM_001685, NM_001320266, NM_001320267
• Synonyms: ATP5; ATP5A; ATPM; CF6; F6
• Summary: 'Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]'