Human Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) activation kit by CRISPRa

CAT#: GA100816

CHRNA7 CRISPRa kit - CRISPR gene activation of human cholinergic receptor nicotinic alpha 7 subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CHRNA7
• Locus ID: 1139
• Kit Components:

  GA100816G1, Nicotinic Acetylcholine Receptor alpha 7 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100816G2, Nicotinic Acetylcholine Receptor alpha 7 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100816G3, Nicotinic Acetylcholine Receptor alpha 7 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000746, NM_001190455, NR_046324, N54079
• Synonyms: CHRNA7-2; NACHRA7
• Summary: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]