Human Collagen IX (COL9A1) activation kit by CRISPRa

CAT#: GA100911

COL9A1 CRISPRa kit - CRISPR gene activation of human collagen type IX alpha 1 chain

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: COL9A1
• Locus ID: 1297
• Kit Components:

  GA100911G1, Collagen IX gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100911G2, Collagen IX gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100911G3, Collagen IX gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001851, NM_078485
• Synonyms: DJ149L1.1.2; EDM6; MED; STL4
• Summary: 'This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'