Human Cytochrome P450 2E1 (CYP2E1) activation kit by CRISPRa

CAT#: GA101104

CYP2E1 CRISPRa kit - CRISPR gene activation of human cytochrome P450 family 2 subfamily E member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CYP2E1
• Locus ID: 1571
• Kit Components:

  GA101104G1, Cytochrome P450 2E1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101104G2, Cytochrome P450 2E1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101104G3, Cytochrome P450 2E1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000773
• Synonyms: CPE1; CYP2E; P450-J; P450C2E
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]'