Human Doublecortin (DCX) activation kit by CRISPRa

CAT#: GA101156

DCX CRISPRa kit - CRISPR gene activation of human doublecortin

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DCX
• Locus ID: 1641
• Kit Components:

  GA101156G1, Doublecortin gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101156G2, Doublecortin gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101156G3, Doublecortin gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000555, NM_001195553, NM_178151, NM_178152, NM_178153, NM_001369374, NM_001369370, NM_001369371, NM_001369372, NM_001369373
• Synonyms: DBCN; DC; LISX; SCLH; XLIS
• Summary: 'This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]'