Human CYB5R3 activation kit by CRISPRa

CAT#: GA101197

CYB5R3 CRISPRa kit - CRISPR gene activation of human cytochrome b5 reductase 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CYB5R3
• Locus ID: 1727
• Kit Components:

  GA101197G1, CYB5R3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101197G2, CYB5R3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101197G3, CYB5R3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000398, NM_001129819, NM_001171660, NM_001171661, NM_007326
• Synonyms: B5R; DIA1
• Summary: 'This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]'