Human Factor IX (F9) activation kit by CRISPRa

CAT#: GA101503

F9 CRISPRa kit - CRISPR gene activation of human coagulation factor IX

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: F9
• Locus ID: 2158
• Kit Components:

  GA101503G1, Factor IX gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101503G2, Factor IX gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101503G3, Factor IX gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000133, NM_001313913
• Synonyms: FIX; HEMB; P19; PTC; THPH8
• Summary: 'This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]'