Human Glycerol 3 Phosphate Dehydrogenase (GPD1) activation kit by CRISPRa

CAT#: GA101884

GPD1 CRISPRa kit - CRISPR gene activation of human glycerol-3-phosphate dehydrogenase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: GPD1
• Locus ID: 2819
• Kit Components:

  GA101884G1, Glycerol 3 Phosphate Dehydrogenase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101884G2, Glycerol 3 Phosphate Dehydrogenase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101884G3, Glycerol 3 Phosphate Dehydrogenase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001257199, NM_005276
• Synonyms: GPD-C; GPDH-C; HTGTI
• Summary: 'This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]'