Human G protein coupled receptor 30 (GPER1) activation kit by CRISPRa

CAT#: GA101914

GPER1 CRISPRa kit - CRISPR gene activation of human G protein-coupled estrogen receptor 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: GPER1
• Locus ID: 2852
• Kit Components:

  GA101914G1, G protein coupled receptor 30 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101914G2, G protein coupled receptor 30 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101914G3, G protein coupled receptor 30 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001031682, NM_001039966, NM_001098201, NM_001505
• Synonyms: CMKRL2; FEG-1; GPCR-Br; LERGU; LERGU2; MGC99678
• Summary: 'This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function. [provided by RefSeq, Aug 2017]'