Human Glutathione Peroxidase 2 (GPX2) activation kit by CRISPRa

CAT#: GA101936

GPX2 CRISPRa kit - CRISPR gene activation of human glutathione peroxidase 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: GPX2
• Locus ID: 2877
• Kit Components:

  GA101936G1, Glutathione Peroxidase 2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101936G2, Glutathione Peroxidase 2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101936G3, Glutathione Peroxidase 2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002083, NR_046320, NR_046321, NR_138078
• Synonyms: GI-GPx; GPRP; GPRP-2; GPx-2; GPx-GI; GSHPx-2; GSHPX-GI
• Summary: 'The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]'