Human HSD17B1 activation kit by CRISPRa

CAT#: GA102247

HSD17B1 CRISPRa kit - CRISPR gene activation of human hydroxysteroid 17-beta dehydrogenase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HSD17B1
• Locus ID: 3292
• Kit Components:

  GA102247G1, HSD17B1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102247G2, HSD17B1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102247G3, HSD17B1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000413, NM_001330219, NR_144397
• Synonyms: EDH17B2; EDHB17; HSD17; SDR28C1
• Summary: 'This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]'