Human IREB2 activation kit by CRISPRa

CAT#: GA102451

IREB2 CRISPRa kit - CRISPR gene activation of human iron responsive element binding protein 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: IREB2
• Locus ID: 3658
• Kit Components:

  GA102451G1, IREB2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102451G2, IREB2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102451G3, IREB2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001320941, NM_001320942, NM_001320943, NM_004136, NM_001354994
• Synonyms: ACO3; IRP2; IRP2AD
• Summary: 'The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]'