Human MBNL1 activation kit by CRISPRa

CAT#: GA102832

MBNL1 CRISPRa kit - CRISPR gene activation of human muscleblind like splicing regulator 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MBNL1
• Locus ID: 4154
• Kit Components:

  GA102832G1, MBNL1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102832G2, MBNL1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102832G3, MBNL1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001314057, NM_021038, NM_207292, NM_207293, NM_207294, NM_207295, NM_207296, NM_207297, NM_001363870
• Synonyms: EXP; EXP35; EXP40; EXP42; MBNL
• Summary: 'This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities. [provided by RefSeq, Sep 2015]'