Human NDUFA1 activation kit by CRISPRa

CAT#: GA103124

NDUFA1 CRISPRa kit - CRISPR gene activation of human NADH:ubiquinone oxidoreductase subunit A1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NDUFA1
• Locus ID: 4694
• Kit Components:

  GA103124G1, NDUFA1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103124G2, NDUFA1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103124G3, NDUFA1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004541
• Synonyms: CI-MWFE; MWFE; ZNF183
• Summary: 'The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]'