Human NFYA activation kit by CRISPRa

CAT#: GA103201

NFYA CRISPRa kit - CRISPR gene activation of human nuclear transcription factor Y subunit alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NFYA
• Locus ID: 4800
• Kit Components:

  GA103201G1, NFYA gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103201G2, NFYA gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103201G3, NFYA gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002505, NM_021705
• Synonyms: CBF-A; CBF-B; HAP2; NF-YA
• Summary: 'The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]'