Human LIS1 (PAFAH1B1) activation kit by CRISPRa

CAT#: GA103370

PAFAH1B1 CRISPRa kit - CRISPR gene activation of human platelet activating factor acetylhydrolase 1b regulatory subunit 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PAFAH1B1
• Locus ID: 5048
• Kit Components:

  GA103370G1, LIS1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103370G2, LIS1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103370G3, LIS1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000430
• Synonyms: LIS1; LIS2; MDCR; MDS; NudF; PAFAH
• Summary: 'This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009]'