Human PCK1 activation kit by CRISPRa

CAT#: GA103414

PCK1 CRISPRa kit - CRISPR gene activation of human phosphoenolpyruvate carboxykinase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PCK1
• Locus ID: 5105
• Kit Components:

  GA103414G1, PCK1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103414G2, PCK1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103414G3, PCK1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002591
• Synonyms: PEPCK-C; PEPCK1; PEPCKC
• Summary: 'This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]'